Easy and efficient agrobacterium tumefaciens induced corn gene transfer method

Abstract

Way to change the genetic of corn with farming bacilli. The character is using the farming bacilli with other genetic to infect the female young fringe and flower of corn take transform of the corn. The transform of the corn is damnificated the flower and female young fringe of the corn first, then put the farming bacilli with objective genetic lay on or dripping on it, repeat it 3 to 4 times, through the filter of fastness and examine of molecule to the seminal, obtain the plant with changed gene. This invention avoid the tissue culture and reformat process of the plant, is simple and short cut, the inverted offspring can individual plant directly.

Description

Technical field: [0001] The present invention relates to a method of transgenic maize. Background technique: [0002] The application of transgenic technology to introduce the target gene into suitable recipients to obtain stable transgenic plants is an important part of transgenic. In maize transgenic research, a variety of transgenic technologies have been established, including gene gun method, PEG method, electric shock method, ultrasonic-mediated method, pollen tube passage method, etc., which are adapted to different receptors and have achieved certain results. However, these technologies have various defects in different aspects such as tissue culture, receptor selection, transformation efficiency, etc., which brings a lot of inconvenience to the experimental operation. [0003] As we all know, the workload of tissue training is relatively large and requires a large number of professionals to engage in heavy work. The traditional Agrobacterium method is based on tissue cul...

AI Technical Summary

Problems solved by technology

At the same time, the tissue culture and callus differentiation of maize are more difficult than those of rice, wheat and other plants. Maize tissue culture is more restricted by genotype and the range of receptors is smaller. The application of gun method, ultrasonic wave and electric shock method on maize has been greatly limited, and although the pollen tube channel method can avoid the tissue culture process and directly obtain transformed seeds, the transformation efficiency is low. Therefore, it is not necessary to establish and develop a new method. Simple and efficient maize transgenic technology through tissue culture is imminent