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Automatic liquid exchange and germ plasma amplifying system between cells or tissues of land plant and seaweed

A technology for plants and algae, applied in biochemical cleaning devices, enzymology/microbiology devices, tissue cell/virus culture devices, etc., can solve the problem of not being able to ensure that the growth environment of the culture is constant and cannot be opened at any time Or shut down the supply of culture medium, cannot provide constant concentration of high-quality seed solution at any time, etc., to reduce the chance of external pollution, enhance the flexibility of control, and simplify the process of germplasm amplification

Inactive Publication Date: 2006-12-13
SHANGHAI JIAOTONG UNIV
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AI Technical Summary

Problems solved by technology

The setup also ignores the lighting system that is critical to cultivating plant and algae cells or tissues, making it difficult for the culture to grow properly, let alone stabilize the cell concentration
In addition, the biggest defect of this system is that it cannot turn on or off the supply of culture solution at any time according to the change of seed solution concentration in the culture tank, nor can it provide constant concentration of high-quality seed solution at any time
All in all, there are defects in the design of this system, that is, the device cannot ensure the constant growth environment of the culture, which is not conducive to its stable cultivation, and cannot provide excellent germplasm resources at any time
This incomplete design will consume extra labor and material resources, and more importantly, the excellent germplasm resources that cannot be cultivated by this system can be used for industrial needs

Method used

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  • Automatic liquid exchange and germ plasma amplifying system between cells or tissues of land plant and seaweed
  • Automatic liquid exchange and germ plasma amplifying system between cells or tissues of land plant and seaweed
  • Automatic liquid exchange and germ plasma amplifying system between cells or tissues of land plant and seaweed

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Embodiment Construction

[0017] Such as Figure 1-4 As shown, the present invention includes: a gas delivery system 1, a culture solution storage system 2, an automatic rehydration pump 3, a controller 4, a seed preservation culture device 5, a peristaltic pump 6, a constant temperature water bath pot 7, a water bath inlet pipe 8, and a water bath outlet pipe 9. Seed taking storage tank 10 , first ventilation pipe 11 , first 0.22 μm microporous membrane filter 12 and seed liquid release switch opening 13 . The connection method is as follows: the gas delivery system 1 and the seed preservation culture tank 5 are connected by a silicone gas pipeline; the culture solution storage system 2 and the seed preservation culture tank 5 are connected by a silicone tube, and the automatic rehydration pump 3 is placed between the two to operate , and connected to the controller 4; the constant temperature water bath pot 7 is connected to the water bath outlet pipe 9 and the water bath inlet pipe 8 (the water bath...

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Abstract

An auto exchange culture and expand system of the land plant with the cell or organism of alga is made up of air transportation system, storage system, auto feeding pump, controller, peristaltic pump, culture equipment, thermostat water bath, pipes, storage pool, first aeration pipe, first filtering film of 0.22um and switch. The system can reach the automated condition.

Description

technical field [0001] The invention relates to an automatic liquid exchange and germplasm amplification device, in particular to an automatic liquid exchange and germplasm amplification device for terrestrial plant and seaweed cells or tissues. Used in the field of cell engineering. Background technique [0002] The industry of using bioreactors to cultivate spirulina has a long history in my country, but the bioreactor cultivation of macroalgae cells and tissues is still in the research stage. Both terrestrial plants and seaweeds require photosynthesis in their growth process. In industry, photobioreactors are generally used for suspension culture, and the proliferation and preservation of cells or tissues is an extremely important technical link. When carrying out small-scale and pilot-scale experiments, a large amount of experimental materials are needed, and when large-scale cultivation in reactors is carried out, a large amount of high-quality germplasm resources are ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M3/00
CPCC12M27/02C12M41/22C12M31/10C12M29/26C12M21/02
Inventor 齐瀚实董良陈思晔张宝红赵锐
Owner SHANGHAI JIAOTONG UNIV
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