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Preparing method for clinical detection reagent

A technology for detecting reagents and anticoagulants, which is applied in the field of preparation of clinical testing reagents, and can solve the problems affecting the determination of turbidimetric quantitative method, the specimen contains color or turbidity, and cannot be used for clinical testing of bacterial endotoxin and colored drugs.

Inactive Publication Date: 2007-01-03
丁友玲
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, in the bacterial endotoxin detection method, the limulus reagent of the gel method can only semi-quantify the bacterial endotoxin in the sample; Bacterial endotoxin in body fluid specimens, because these specimens contain interfering substances, the specimens contain color or turbidity, which affects the determination of turbidity quantitative method
Therefore, the original Limulus reagent cannot be used for clinical testing of bacterial endotoxins and colored drugs in various blood and body fluid specimens.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0008] After blood collection with live limulus plus anticoagulant, centrifuge at 1000rpm, discard supernatant, collect cells, then add washing solution containing NaCl 3mol / L and Tris HCl 0.05mol / L pH 8.0 to wash, discard supernatant; collect Add the disintegration solution containing NaCl 0.1mol / L and Tris HCl 0.02mol / L pH 8.0 to disintegrate the cells, and then centrifuge to collect the extract; Wash the column with Tris-HCl eluent, collect the eluent; after identifying the eluent, combine the eluent with the same composition, and concentrate at low temperature with a freeze dryer; the concentrated solution is re-dissolved and desalted. And collect the eluate without salt; add 10ml of 0.5mg / mL protease protectant (human albumin) to the eluate without salt, and then carry out low-temperature concentration; after redissolving the concentrate, add 1.0mg / mL 4ml of mlBoc-LGR-pNA chromogenic substance and 10ml of 0.5mg / ml protease protective agent were mixed evenly, then subpacka...

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Abstract

It is a clinic test agent process method, which comprises the following steps: to take the blood sample of live horsefoot by joining the anticoagulant; to take the horsefoot cells to do the affinity chromatography after cell breakdown; to collect eluents with different salt condensation; and to integrate the eluents with same component for low temperature compression and solve and salt-isolation; to join the protective agent in low temperature and compression; to mix the chromophoric material and protective agent; to divide the liquid and to dry and seal it to get the test agent. The test agent is mixed with the Tris-HCI buffer and the nitridizing agent to make clinic test agent box to be used in the ration measurement of the poison in bacteria.

Description

technical field [0001] The invention relates to a preparation method of a clinical detection reagent. Background technique [0002] At present, in the bacterial endotoxin detection method, the limulus reagent of the gel method can only semi-quantify the bacterial endotoxin in the sample; When bacterial endotoxins are contained in body fluid specimens, these specimens contain interfering substances, which cause the specimens to contain color or turbidity, which affects the determination of the turbidimetric method. Therefore, the original limulus reagent cannot be used for clinical examination of bacterial endotoxins and colored medicines in various blood and body fluid specimens. Contents of the invention [0003] The purpose of the present invention is to provide a preparation method of a clinical detection reagent, the finished product of which can be used for more precise, accurate and rapid detection of bacterial endotoxin. [0004] The preparation method of a kind o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50G01N33/579
Inventor 丁友玲
Owner 丁友玲