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Hydrophobically-modified protein compsns, and methods

A hydrophobic group and protein technology, which is applied in the preparation method of peptides, drug combinations, peptide/protein components, etc., can solve the problems of negative impact on the application of preparations and complicated experiments

Inactive Publication Date: 2001-05-09
生物基因公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, protectants also have significant biological activity themselves, and they thus have the potential to complicate experiments and negatively impact the therapeutic application of the formulation

Method used

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  • Hydrophobically-modified protein compsns, and methods
  • Hydrophobically-modified protein compsns, and methods
  • Hydrophobically-modified protein compsns, and methods

Examples

Experimental program
Comparison scheme
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Embodiment approach

[0012] Another embodiment is an isolated protein of the form A-Cys-[Sp]-B-X, wherein A is a hydrophobic group, Cys is cystine or its functional equivalent, [Sp] is an optional spacer peptide sequence, and B is A protein comprising a plurality of amino acids, including at least one optional spacer peptide sequence; and X is an optional other hydrophobic group attached to the protein.

[0013] The isolated protein may be an extracellular signaling protein, preferably a hedgehog protein. The protein may be modified with at least one hydrophobic group at at least one other amino acid. In another embodiment, the protein is in contact with a vesicle selected from the group consisting of cell membranes, micelles and liposomes.

[0014] Another aspect of the invention is an isolated protein having a C-terminal amino acid and an N-terminal thioproline group formed by the reaction of an aldehyde with the N-terminal cystine of the protein . Yet another aspect of the invention is an is...

Embodiment 4

[0176] Preferred sterols and sterol esters suitable for use in preparing multimeric protein complexes include cholesterol, cholestanol, cholesterol sulfate, and other cholesterol analogs and derivatives. The fact that vesicles can comprise many different lipids and detergents allows for great variability in engineering binding protein-vesicle complexes with desired properties. For example, one can make vesicles that incorporate different kinds of proteins by changing the composition of the lipids in the starting material to produce larger vesicles, or by increasing the percentage of phosphatidylinositol lipids in the vesicles. Ⅶ. Practicality

[0177] In general, the modified proteins described herein can be used to treat conditions that can be treated with unmodified proteins. However, the hydrophobically modified proteins described herein have several significant improvements over the unmodified form. First, their increased potency allows smaller amounts of protein to be u...

Embodiment 1

[0194] The invention will now be described in more detail with reference to the following non-limiting examples. Example 1: Lipid modification of human Sonic hedgehog when expressed in insect cells A. Expression of human Sonic hedgehog

[0195] The cDNA of full-length human Sonic hedgehog (Shh) was subcloned in pBluescript SK + The 1.6 kb EcoRI fragment from (20) (a gift from David Bumcrot of Ontogeny, Inc., Cambridge MA). The 5' and 3' NotI sites, which immediately flank the Shh open reading frame, were added by unique site-elimination mutagenesis using the Pharmacia kit according to the manufacturer's recommended protocol. The 1.4 kb NotI fragment carrying the full-length Shh cDNA was then subcloned into the insect expression vector pFastBac (Life Technologies, Inc.). Using Life Technologies, Inc. The provided procedure produces recombinant baculovirus. The obtained virus is used to generate a high titer virus stock solution. Methods for the preparation and purification...

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Abstract

The present invention relates to hydrophobically modified proteins and methods for their preparation. Hydrophobic groups are attached to the surface amino acid residues of proteins. Hydrophobic groups can be lipids or peptides. Alternatively, the protein can be derivatized by various reactive reactions that suspend hydrophobic structures on the protein. Preferred proteins are mammalian and selected from the group consisting of Sonic, Indian and Desert hedgehog. Hydrophobic groups can be used as convenient linking groups to attach vesicles such as cell membranes, liposomes or micelles.

Description

Background of the invention [0001] It is well known that certain proteins, when attached to other moieties, either by forming multimeric complexes in which the proteins have the opportunity to function in association, or by other changes in the physico-chemical properties of proteins such as protein absorption, biodistribution and half-life, May have higher biological activity. Thus, a major current area of ​​biotechnology research involves the development of methods to modify the physico-chemical properties of proteins so that they can be administered in smaller quantities, with fewer side effects, new routes and at lower cost. [0002] For example, the binding affinity (eg, the affinity of a ligand for its cognate receptor) of any individual protein is likely to be low. However, cells normally express hundreds to thousands of replicas of a particular surface receptor, and many receptor-ligand interactions occur simultaneously. When many surface molecules participate in the...

Claims

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Application Information

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IPC IPC(8): A61K38/00A61K38/17A61K38/21A61K38/22A61K38/27A61K38/28A61P25/00A61P43/00C07K1/107C07K14/00C07K14/46
CPCC07K14/46C07K1/1077A61K38/1709C07K2319/00A61P25/00A61P43/00C07K14/435
Inventor R·布莱克·佩平斯基达伦·P·贝克闻定一凯文·P·威廉斯埃伦·A·加伯弗雷德里克·R·泰勒阿方斯·加尔德斯杰弗里·波特
Owner 生物基因公司
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