Method, apparatus and compositions for inhibiting the human scent tracking ability of mosquitoes in environmentally defined three dimension spaces
A composition and environmental technology, applied in the fields of botanical equipment and methods, biocides, animal repellants, etc., can solve problems such as not being very effective
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Embodiment 1
[0024] The three-cage and two-mouth odor analyzer described and described in "Journal of Medical Entomology" [J.Med.Entomol.], 35(3), 330-334(1998) by Posey et al. was used to study 6-8 Reaction of day-old laboratory-bred adult female Aedes aegypti. This system allows mosquitoes to choose between two different stimuli. The odor tester is made of transparent acrylic plastic and contains three test chambers in a layered configuration, each with a pair of movable sleeves and mosquito traps. The instrument is equipped with a filtered external air supply system, which can accurately control temperature (+ / -0.5°C) and relative humidity (+ / -2%). Only one room is used for testing at a time. Before entering the selection port, mosquito trap and odor detector, the outside air must pass through a series of carbon filters and, if necessary, be heated and humidified for air conditioning.
[0025] One hour before the start of the experiment, approximately 75 female Aedes aegypti mosquitoes were...
Embodiment 2
[0031] The test compound was used together with the synthetic human odor attractant, and the experiment was carried out similar to that described in Example 1. The combination of experiments is as follows:
[0032] (1) CS vs. CS (comparison)
[0033] (2) Dehydrolinalool + CS vs. CS
[0034] (3) Linalool + CS vs. CS
[0035] For comparison, the well-known tropical mosquito repellent, DEET-N,N-dihexyl-3-methylbenzamide is used as a control for dehydrolinalool and linalool. The test concentration of each compound is 100, 250 and 500 μl, respectively. The results are listed in Tables 2, 3, and 4. The data in the table is still expressed in terms of the percentage of mosquitoes attracted to mouth 1 or 2, and the percentage of mosquitoes that were not attracted to mouth 1 or 2.
[0036] Test compound and dosage
[0037] Test compound and dosage
[0038] Test compound and dosage
Embodiment 3
[0040] In the further inhibitory ability test of the compound of the present invention, the following field test was carried out using linalool as an inhibitor. The experiment was set up in Sarasota, Florida, and the experiment site was adjacent to the wetland breeding area where the forest was planted. 200ml / min CO for trap 2 As a bait, it is equivalent to the breath of a person weighing 91 kg (2001b). As an experimental control, it is used continuously every night. In the collection of the trap, the mosquito population is counted first, and then the type of mosquito is checked. About 91% of the mosquito population are Culex nigripalpus. In Florida, it is the vector of St. Louis encephalitis.
[0041] The experimental design uses a 2×2 Latin square. The experiment was carried out in two open areas separated by 24 meters (80 ft), marked as positions 1 and 2, respectively. Each area is 2.4 meters × 2.4 meters (8ft × 8ft), which is 5.76 square meters (64ft 2 ). The wooden pillars ar...
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