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Wheat sterile apicule number QTL mapping inter-block

A technology of spikelet number and wheat, applied in the fields of plant genetic improvement, microbial measurement/inspection, botany equipment and methods, etc.

Inactive Publication Date: 2007-08-15
CROP BREED RESOURCES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The number of sterile spikelets in wheat is a reflection of the genotype's ability to adapt to drought conditions, and there is no report on the QTL mapping of the number of sterile spikelets in wheat under drought conditions

Method used

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  • Wheat sterile apicule number QTL mapping inter-block

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0013] Example: Establishment of Chromosomal Mapping Interval Xgwm260-WMC83-WMC301 of Wheat Sterile Spikelet Number QTL

[0014] Two common wheat cultivars Hanxuan 10 and Lumai 14 were used to prepare a hybrid combination. 1 The anthers were cultured to obtain haploid plants, and after doubling, 221 doubled haploid (DH) plants were obtained, and a genetic mapping population was created.

[0015] A molecular genetic linkage map with 395 markers was constructed using the DH population, including SSR and AFLP. The PCR experimental procedures for SSR markers refer to the method of Rder et al. (Genetics, 1998, 149: 2007-2023); the AFLP markers are digested with PstI and MseI respectively, and the experimental procedures refer to Vos et al. (Nucleic Acids Res, 1995, 23:4407-4414) approach. AFLP and SSR-labeled PCR products were detected by 6% polyacrylamide gel electrophoresis, and the experimental procedures were referred to the method of Rder et al. (Genetics, 1998). The mole...

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Abstract

A chromosome mapping interval Xgwm 260-WMC83-WMC301 for the sterile spikelet number QTL of wheat is disclosed, where the WMC 83 may be used as the molecular marker for selecting the sterile spikelet number of wheat. It can be used to culture the drought-resistant wheat.

Description

technical field [0001] The invention belongs to the field of molecular breeding, and in particular relates to a QTL mapping interval for the number of sterile spikelets of wheat under drought conditions. Background technique [0002] Drought and water shortage is a serious problem faced by global agricultural production, and it is also an important factor restricting the development of my country's grain production. Wheat is the main food crop of human beings. At least 60 million hectares of wheat are cultivated in rain-fed arable land in developing countries all over the world, and the yield level is only 10%-50% of that under irrigation conditions. my country's average water requirement for producing 1 ton of wheat is about 500-700m 3 . Evidence shows that planting drought-resistant and water-saving varieties can significantly improve the water use efficiency of crops. Therefore, cultivating drought-resistant and water-saving varieties is the most economical and effecti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68A01H1/00C12Q1/04A01H1/04
Inventor 景蕊莲昌小平贾继增
Owner CROP BREED RESOURCES INST CHINESE ACAD OF AGRI SCI