Circulation and physiological stress simulating engineering tissue three-dimensional cultivation device

Abstract

The three-dimensional cultivation device is provided with new culture liquid storing bottle, circulating culture, liquid storing bottle, 3D tissue cell cultivation chamber and waste liquid collectingbottle and among them there are silica duct, liquid for drive, pressure inhibitor and gas flow valve connected electrically to culture liquid renewing automatic controller, stress regulating detectorand gas flow rate controller separately. Inside the 3D tissue cell cultivation chamber, there is bearing net with engineering tissue organ and hydraulic sensor connected electrically to stress regulating detector. The present invention cultures engineered tissue and organ in an environment with simulated blood circulation and physiological stress suitable for tissue growth and maturation, and theautoamtic process has less work amount.

Description

1. Technical field [0001] The invention relates to a tissue cell culture equipment, in particular to a three-dimensional culture device for simulating engineering tissue of circulation and physiological stress. 2. Background technology [0002] Tissue engineering is the application of the principles of life science and engineering. Seed cells are connected to a shaped three-dimensional mesh scaffold material, and then cultured in vitro to generate tissues and organs with specific functions, which are used for the repair of tissues and organs in the body. It is difficult to meet the needs of the development of tissue engineering by using traditional culture methods such as culture plates, culture dishes, and culture bottles to change the medium regularly, because the need to change the medium every time not only destroys the steady state of the biochemical microenvironment during the culture process, but also discards the secretion of the cells. and the effective distance of ...

AI Technical Summary

Problems solved by technology

It is difficult to meet the needs of the development of tissue engineering by using traditional culture methods such as culture plates, culture dishes, and culture bottles to change the medium regularly, because the need to change the medium every time not only destroys the steady state of the biochemical microenvironment during the culture process, but also discards the secretion of the cells. and the effective distance of one-way diffusion to maintain activity in static cultur

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