Use of a class of enzymes and their encoding genes to increase the oil content in transgenic organisms

A transgenic and biological technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problem of not explaining Are1 and so on

Inactive Publication Date: 2003-02-26
BASF PLANT SCI GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the prior art does not indicate that Are1 is a TAG synthase, nor is it possible to conclude that Are1 is a DAGAT based on published homology to ACAT-like sequences (Lassner and Ruezinsky, 1999)

Method used

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  • Use of a class of enzymes and their encoding genes to increase the oil content in transgenic organisms
  • Use of a class of enzymes and their encoding genes to increase the oil content in transgenic organisms

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Embodiment 1

[0031] SEQ ID NO.2: Amino acid sequence of Saccharomyces cerevisiae open reading frame YCR048W. Example Example 1 - Reduced triacylglycerol accumulation in yeast cells lacking the ARE1 gene Materials and methods

[0032] yeast strain. The yeast strain used in this study was congenic to the W303-1A (Thomas and Rothstein, 1989) background. By making two strains SCY60 (MATaare1-Δ::HIS3 ade2-1 can1-100 leu2-3, 112 trp1-1 ura3-1) and SCY61 ​​(MATaare2-Δ::LEU2 ADE2 can1-100 his3-11, 15 trp1- 1 ura3-1) (Yang et al., 1996) were crossed, followed by separation of tetrads, resulting in the are1 mutant H1111 with the genotype MATαare1-Δ::HIS3 ADE2can1-100 leu2-3, 112 trp1-1 ura3-1. As a wild-type control, we used SCY62 (MATa ADE2 can1-100 his3-11, 15 leu2-3 trp1-1 ura3-1) (Yang et al., 1996). Using the lithium acetate method, through a series of yeast transformations, yeast mutants with disrupted ARE1 genes in YNR008w and YOR245c encoding yeast DAGAT B and PDAT, respectively, were cons...

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Abstract

The present invention relates to the use of a novel enzyme and its encoding gene for transformation. More specifically, the invention relates to the use of a gene encoding an enzyme with acyl-Co A: diacylglycerol acyltransferase activity. This gene expressed alone in transgenic organisms will increase the total amount of oil, i.e. triacylglycerols, that is produced.

Description

field of invention [0001] The present invention relates to a novel enzyme and the use of its coding gene for transformation. More specifically, the present invention relates to the use of a gene encoding an enzyme having acyl-CoA:diacylglycerol acyltransferase activity. Expression of this gene alone in a transgenic organism will increase the total amount of oil (ie, triacylglycerol) produced. Background of the invention [0002] In oil crops like canola, sunflower, oil palm, etc., oil (ie triacylglycerol) is the most valuable product in the seeds or fruits, while other compounds such as starch, protein and fiber are considered as low value by-products. Increasing the quantity per weight of oil in an oil crop by consuming other compounds will thus increase the value of the crop. If the enzymes that regulate the partitioning of reduced carbon into oil production can be upregulated by overexpression, the cell will accumulate more oil by consuming other products. Such methods...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C12N9/10C12N15/54C12N15/82C12P7/64
CPCC12P7/6463C12N15/8247C12N9/1029C11B1/00
Inventor A·巴纳斯L·桑达格尔U·斯塔尔A·达尔奎斯特M·伦曼H·龙纳S·斯蒂姆尼
Owner BASF PLANT SCI GMBH
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