Quick screening method of alkali resistance rice breeding material

A screening method and alkali resistance technology, which are applied in the field of rapid screening of alkali resistance rice breeding materials, can solve the problems of being unsuitable for alkali resistance research, laborious and time-consuming, etc.

Inactive Publication Date: 2003-04-30
CHANGCHUN INST OF GEOGRAPHY CHINESE ACAD OF SCI
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Problems solved by technology

[0004] Field screening is suitable for the identification of salt tolerance of plants, but it is not suitable for the study of alkali resistance, which is time-consuming and laborious
[0005] Indoor screening, suitable for salt tolerance identification of plants, not suitable for alkali resistance research

Method used

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Examples

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Embodiment

[0019] The test materials used rice varieties Nipponbare and Kasalath as controls, and 98 offspring lines from Nipponbare and Kasalath. Choose 5mM, 10mM, 15mM and 20mM four concentrations of Na 2 CO 3 Firstly, preliminary identification of alkali resistance was carried out on the parents Nipponbare and Kasalath. Extensive screening was then performed using standard methods as follows. That is to say, the seeds are immersed in 0.2% "Ben Leite" disinfectant solution, and placed in a 30°C thermostat for high-temperature sterilization for 24 hours. Wash with tap water 4-5 times, and then place it in a 30°C incubator for 36 hours at high temperature to accelerate germination. The germinated seeds were sown on special nylon nets, and moved into a controlled greenhouse at 30°C with tap water for 7 days. Then it was transferred to the Kimura pre-nutrient solution with a concentration of 1 / 2C, and pre-cultured for 7 days before treatment in a controlled greenhouse at 30°C. Then, u...

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Abstract

A method for quickly screening the alkali-resistance paddy rice includes such steps as choosing 2-4 standard refrerence varieties including at least one pair of sensitive and insensitive varieties, sterilizing, accelerating germination, sowing, pre-culturing, alkali stress treating, restorative growth and evaluating the alkali damage. It can be used for screening, genetic analysis and alkali-resistant functional gene mapping.

Description

Technical field: [0001] The invention belongs to the variety breeding technology in the field of agriculture, relates to a selection method for selecting new plant varieties suitable for cultivation in salinized soil, and is a rapid screening method for alkali-resistant rice breeding materials. Background technique: [0002] The global surface has 1.05 billion hm 2 of salinized soil, of which 230 million hm 2 It is non-intensive salinized soil with production possibility. China has a huge area of ​​saline-alkali soil, which can be divided into saline-alkali wasteland and saline-alkali cultivated land according to its utilization. Among them, the saline-alkali wasteland is about 20 million hm 2 , about 6.67 million hm of saline-alkali cultivated land 2 . About 10% of the irrigated farmland has secondary salinization or is in danger of secondary salinization, and about 80% of the saline soil has not been effectively developed and utilized. Therefore, the development and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04
Inventor 梁正伟王志春
Owner CHANGCHUN INST OF GEOGRAPHY CHINESE ACAD OF SCI
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