Quick screening method of alkali resistance rice breeding material
A screening method and alkali resistance technology, which are applied in the field of rapid screening of alkali resistance rice breeding materials, can solve the problems of being unsuitable for alkali resistance research, laborious and time-consuming, etc.
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[0019] The test materials used rice varieties Nipponbare and Kasalath as controls, and 98 offspring lines from Nipponbare and Kasalath. Choose 5mM, 10mM, 15mM and 20mM four concentrations of Na 2 CO 3 Firstly, preliminary identification of alkali resistance was carried out on the parents Nipponbare and Kasalath. Extensive screening was then performed using standard methods as follows. That is to say, the seeds are immersed in 0.2% "Ben Leite" disinfectant solution, and placed in a 30°C thermostat for high-temperature sterilization for 24 hours. Wash with tap water 4-5 times, and then place it in a 30°C incubator for 36 hours at high temperature to accelerate germination. The germinated seeds were sown on special nylon nets, and moved into a controlled greenhouse at 30°C with tap water for 7 days. Then it was transferred to the Kimura pre-nutrient solution with a concentration of 1 / 2C, and pre-cultured for 7 days before treatment in a controlled greenhouse at 30°C. Then, u...
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