Immunity colloidal gold reagent for detecting IgG antibody of herpes simplex virus I type and its preparing method

Abstract

The reagent includes the sample pad, the binding pad, the cellulose nitrate film, the water uptake pad and the PVC back cover. The sample pad, the binding pad are adhered to the end with PVC back covered in sequence. The cellulose nitrate film is adhered to the middle. The water uptake pad is adhered to another end of the reagent. The binding pad is coated with the label of albumen A (or rabbit anti human IgG)-colloidal gold. The cellulose nitrate film is coated with the specifity surface film antigen of herpes simple virus I type and chickens anti albumen A (or sheep anti rabbit IgG). The reagent possesses the features of the high specificity and sensitivity, simple and fast as well as the low cost. The reagent is applicable to the detection in site within 30 min for the full testing procedure without need of professional training for the operation personnel.

Description

technical field [0001] The invention relates to an immune colloidal gold reagent for detecting antibodies, and also relates to a preparation method of the reagent. Background technique [0002] Existing methods for detecting IgG of herpes simplex virus type I mainly include enzyme-linked immunosorbent assay, radioimmunoassay, immunocolloidal gold diafiltration, and Western blotting (WBA). The disadvantages of the enzyme-linked immunosorbent (EIA) method are: special instruments and equipment such as microplate readers are required for use; detection operators need to undergo professional training; the operation process is relatively complicated, and the time required for detection is relatively long; the cost of detection Higher, single-person detection cannot be realized. The disadvantages of immunocolloidal gold diafiltration are: the operation process is relatively complicated, and one-step operation cannot be realized; reagents need to be stored a...

AI Technical Summary

Problems solved by technology

The disadvantages of the enzyme-linked immunosorbent (EIA) method are: special instruments and equipment such as microplate readers are required for use; detection operators need to undergo professional training; the operation process is relatively complicated, and the time required for detection is relatively long; the cost of detection High, unable to achieve single-person detection

The disadvantages of immunocolloidal gold diafiltration are: the operation process is relatively complicated, and one-step operation cannot be realized; reagents need to be stored at low temperature; the cost of detection is high; the time required for detection is longer than that of colloidal gold immunochromatography

The disadvantages of radioimmunoassay are: operators need to undergo professional training; the operation process is complex and demanding; reagents need to be stored at low temperature; because the test involves radioactive isotopes, the test is not very safe, so it is rarely used; the test cost is high; takes a long time

The defect of western blotting assay (WBA) is: the operator needs to be through professional training; Limited to some professional laboratories; high cost of testing; long time required for testing