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Method and device for identifying pathogenic influence of concurrent bacteria infection against poultry influenza virus infection

A technology for virus infection and bacterial infection, applied in tissue cell/virus culture devices, biochemical equipment and methods, microbial measurement/inspection, etc., can solve economic and foreign trade losses and other problems, and achieve low price and easy operation Effect

Inactive Publication Date: 2003-09-17
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, even if the laboratory proves that the isolate is of low pathogenicity, the chickens are treated as strictly as highly pathogenic influenza, thus causing great losses to the economy and foreign trade

Method used

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  • Method and device for identifying pathogenic influence of concurrent bacteria infection against poultry influenza virus infection
  • Method and device for identifying pathogenic influence of concurrent bacteria infection against poultry influenza virus infection
  • Method and device for identifying pathogenic influence of concurrent bacteria infection against poultry influenza virus infection

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1: Identify the impact of concurrent bacterial infection on the pathogenicity of viral infection (refer to figure 1 )

[0025] 1. Using a cell homogeneous culture device in vitro, culture CEFs with conventional cell culture medium 4 in 3 Kjeldahl cell culture flasks 1 respectively, and inoculate the avian influenza virus to be identified when the CEFs reach 80% fusion;

[0026] 2. Inoculate Bacillus subtilis and tryptase-like bacteria in solid LB culture dishes, culture at 37°C for 12 hours, and pick a single colony;

[0027] 3. the bacteria of Bacillus subtilis and tryptase are inoculated on the a wall 31 insides of 2 bacterial culture chambers 3 respectively (as figure 2 shown). Then put 2 bacterial culture chambers into the culture solution 4 of the 2 cell culture bottles in step 1 respectively, and leave 1 cell culture bottle only inoculated with avian influenza virus as a control group, continue to cultivate, and observe each cell culture bottle Pres...

Embodiment 2

[0033] Referring to Example 1, TOP-10 bacteria were inoculated in a low-salt solid LB culture dish, cultivated at 37°C for 12 hours, picked a single colony, inoculated TOP-10 bacteria in the bacterial culture room on the A wall, and put them into culture for 80 % confluent WCK cell culture flask, continue to culture and observe whether there are lesions.

Embodiment 3

[0035] Referring to Example 1, inoculate DH5α Escherichia coli on a solid LB petri dish, culture at 37°C for 12 hours, pick a single colony, inoculate DH5α Escherichia coli in the bacterial culture room on wall A, and put it into WDCK that has reached 80% fusion In the cell culture flask, continue to culture and observe whether there are lesions.

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Abstract

A method for determining the affection of concurrent bacterial infection to virus infection pathogenicity includes such steps as inoculating the bacteria and low-pathogenicity virus in cell culturingmedium, culturing, and observing in cell lesion (CPE) presents. Its culture equipment is composed of cell culture bottle and bacteria culture chamber. Its advantages are simple process, short time, high currectness and low cost.

Description

technical field [0001] The invention relates to a method for identifying the influence of concurrent bacterial infection on the pathogenicity of viral infection and a device for homogeneous cell culture in vitro. Background technique [0002] Existing bacterial culture is carried out separately from cell culture. If bacterial contamination occurs in the cell flask during the cell culture process, the cell culture and subculture will not be able to go on for a long time. The main reasons are as follows: a) Bacteria encroach on cells. b) The effects of toxins secreted by bacteria during growth on the cell membrane or different parts of the cell. c) The enzymes secreted by bacteria during the growth process affect a certain link of cell growth. d) During the process of high-speed division, bacteria occupy most of the growth space and absorb a large amount of nutrients, which in turn affects the growth of cells. [0003] In the process of c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12Q1/00
Inventor 杨德威刘福安
Owner SUN YAT SEN UNIV
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