Method and device for identifying pathogenic influence of concurrent bacteria infection against poultry influenza virus infection

A technology for virus infection and bacterial infection, applied in tissue cell/virus culture devices, biochemical equipment and methods, microbial measurement/inspection, etc., can solve economic and foreign trade losses and other problems, and achieve low price and easy operation Effect
CN1442486AInactive Publication Date: 2003-09-17SUN YAT SEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUN YAT SEN UNIV
Publication Date
2003-09-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

A method for determining the affection of concurrent bacterial infection to virus infection pathogenicity includes such steps as inoculating the bacteria and low-pathogenicity virus in cell culturingmedium, culturing, and observing in cell lesion (CPE) presents. Its culture equipment is composed of cell culture bottle and bacteria culture chamber. Its advantages are simple process, short time, high currectness and low cost.
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Description

technical field

[0001] The invention relates to a method for identifying the influence of concurrent bacterial infection on the pathogenicity of viral infection and a device for homogeneous cell culture in vitro. Background technique

[0002] Existing bacterial culture is carried out separately from cell culture. If bacterial contamination occurs in the cell flask during the cell culture process, the cell culture and subculture will not be able to go on for a long time. The main reasons are as follows: a) Bacteria encroach on cells. b) The effects of toxins secreted by bacteria during growth on the cell membrane or different parts of the cell. c) The enzymes secreted by bacteria during the growth process affect a certain link of cell growth. d) During the process of high-speed division, bacteria occupy most of the growth space and absorb a large amount of nutrients, which in turn affects the growth of cells.

[0003] In the process of c...

Claims

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