Process for raising glutathion yield by fermentation of tornla yeast
A technology of Candida utilis and glutathione, applied in the direction of fermentation, etc., to achieve significant social and economic benefits
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[0031] Example 1 The experimental results of preparing glutathione according to the fermentation conditions described in the instructions without adding L-cysteine in the shake flask
[0032] Dry cell weight: 9-10g / L; GSH production: 180-200mg / L; GSH content: 1.8-2.1%.
[0033] The fermentation time for the best results is 24 hours.
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[0034] Example 2 The experimental results of preparing glutathione according to the fermentation conditions described in the specification and adding L-cysteine to the shake flask
[0035] Adding time: 0-20 hours; Adding concentration: 6-10mmol / L;
[0036] Dry cell weight: 9-10g / L; GSH yield: 300-410mg / L; GSH content: 2.0-4.2%.
[0037] The best addition time is 16 hours; the best addition concentration is 8mmol / L.
Example Embodiment
[0038] Example 3 According to the fermentation conditions described in the specification, glutathione was prepared without adding L-cysteine in the 7L fermentor.
[0039] Dry cell weight: 14-16g / L; GSH production: 220-300mg / L; GSH content: 2.0-2.2%.
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