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Process for overcoming self-incompatibility of plant by electrical field

A process method and affinity technology, applied in the field of electric field to overcome plant mating incompatibility, can solve the problems of difficulty in finding the appropriate dose, low probability of success, pollen variation, etc., and achieve the improvement of self-compatibility rate , Affinity rate increase, the effect of increasing affinity rate

Inactive Publication Date: 2003-11-19
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is not easy to find the appropriate dose, often leads to pollen mutation or death, the probability of success is small

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Collect mature black mustard anthers, and place them under ventilated and dry conditions for 1.5-2 hours. When the pollen begins to shed, the pollen is treated with a high-voltage flat-plate electrostatic field, and pollinated on the mature stigma of kale. The selected electric field output voltage is 20KV, the treatment field strength is 200KV / m, the treatment time is 30min, and the hybrid seed setting rate of the control combination is doubled.

Embodiment 2

[0060] Collect mature grassland gentian anthers, and place them under ventilated and dry conditions for 2-3 hours. When the pollen begins to shed, the pollen is treated with a high-voltage flat electrostatic field, and pollinated on the mature stigma. The selected electric field output voltage is 5KV, the treatment field strength is 500V / m, the treatment time is 30min, and the seed setting rate is increased by 20%.

Embodiment 3

[0062] Collect mature lily anthers, and place them under ventilated and dry conditions for 2-2.5 hours. When the pollen begins to shed, the pollen is treated with a high-frequency electric field, and the pollen is pollinated on the mature stigma. The output power of the high-frequency electric field is selected as 20W, and the processing time is 1-3min.

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PUM

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Abstract

A technology for overcoming the mating incompatibility of plant by electric field includes such steps as picking up mature anthers, drying for 1-3 hr until pollen is released, treating the pollen in electric field, and pollinating within 1-5 hr. It can increase the setting percentage by 10-40%.

Description

Technical field [0001] The invention relates to an electric field treatment process method, in particular to a process method for electric field to overcome plant mating incompatibility. Background technique [0002] Self-incompatibility is a common phenomenon in the plant world, and it is a reproductive isolation that is beneficial to cross-pollination formed during the long-term evolution of plants. Self-incompatibility of plants refers to the phenomenon that hermaphroditic plants that can produce male and female gametes with normal functions and mature at the same time cannot complete fertilization during self-pollination or cross-pollination of the same genotype. Self-incompatibility has important theoretical and practical significance in plant reproductive biology and heterosis utilization. [0003] The breeding and utilization of self-incompatible lines is an important discovery in the utilization of heterosis, which can save the labor of manual emasculation, reduce t...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 李玉花丁兵解丽楠于晓艳蓝兴国佟友丽王继刚冯国军
Owner NORTHEAST FORESTRY UNIVERSITY
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