Tissue chip and cellline chip for observation of cell signal conducting path
A signal transduction and cell line technology, applied in the field of tissue and cell chip for high-throughput detection and analysis of signal transduction pathways in different cells, can solve the problem of inability to directly detect the expression changes of one or more genes in situ
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Embodiment 1
[0072] Phosphorylation status detection of Akt gene in normal liver tissue, liver cancer tissue, paracancerous liver tissue and liver cancer cell lines
[0073] 1. Materials and Methods
[0074] Cancer tissue-cell line chip: The tissue chip used in this study consists of 54 pairs of hepatocellular carcinoma and paracancerous tissues, normal liver tissues of 9 cases of accidental death, and subcutaneous tumor tissues of nude mice with 3 hepatoma cell lines Hep3B, SMMC7721, and BEL7402 Equal composition (see Table 1 and figure 1 ).
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QD-01K
++
NPEF
QD-01N
++NP
QD-02K
+++NEF
QD-02N
+++NPEF
QD-03K
++NPE
QD-03N
++NPEF
QD-04K
++NPE
QD-04N
+++NE
+P
QD-05K
+++...
Embodiment 2
[0101] Phosphorylation status detection of Smad1 gene in normal liver tissue, liver cancer tissue, paracancerous liver tissue and liver cancer cell lines
[0102] According to the method described in Example 1, immunohistochemical analysis was performed with antibodies against Smad1 phosphorylated protein and Smad1 total protein.
[0103] The results showed that the phosphorylation of Smad1 in the cytoplasm of cancer cells was weakly positive, the positive rate was 31.37% (16 / 51), and it was negative in the nucleus; while the cytoplasm of paracancerous liver and normal liver tissue were all positive in a moderate degree , the positive rate was 100% (57 / 57; 9 / 9); SMMC7721, BEL7402 and Hep3B showed moderate positive reaction in the cytoplasm.
[0104] The total protein of Smad1 in the cytoplasm of cancer cells was weakly positive, the positive rate was 31.37% (16 / 51), and the nucleus was also positive to varying degrees; while the weakly positive rate in the cytoplasm of adjacen...
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