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Preparation of alpha-1,4-glucan lyase and nucleic acid encoding same

A technology of dextran and lyase, applied to α-1, can solve the problem of not providing the amino acid sequence and achieve the effect of easy preparation

Inactive Publication Date: 2004-03-17
DUPONT NUTRITION BIOSCIENCES APS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the document mentions the GL enzyme, it does not provide any amino acid sequence of the enzyme, let alone any nucleotide sequence data encoding the enzyme

Method used

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  • Preparation of alpha-1,4-glucan lyase and nucleic acid encoding same
  • Preparation of alpha-1,4-glucan lyase and nucleic acid encoding same
  • Preparation of alpha-1,4-glucan lyase and nucleic acid encoding same

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Embodiment Construction

[0054] The invention will now be described by way of example only.

[0055] The sequence data below were used to prepare primers for the PCR reactions described below and to detect the amino acid sequences generated from the respective nucleotide sequences.

[0056] Ammonia assembled from peptides in fungal-infected Gracilariopsis lemaneifomis amino acid sequence : Tyr Arg Trp Gln Glu Val Leu Tyr Thr Ala Met Tyr Gln A5n AlaAla Phe Gly Lys Pro Ile Ile Lys Ala Ala Ser Met Tyr Asn AsnAsp Ser Asn Val Arg Arg Ala Gln Asn Asp His Phe Leu Leu GlyGly His Asp Gly Tyr Arg Ile Leu Cys Ala Pro Val Val Trp GluAsn Ser Thr Glu Arg Glu Leu Tyr Leu Pro Val Leu Thr Gln TrpTyr Lys Phe Gly Pro Asp Phe Asp Thr Lys Pro Leu Glu Gly Ala

[0057] Amino acid sequence (27-34) (Met Tyr Asn Asn Asp Ser Asn Val)

[0058] Primer A: ATG TA(TC) AA(CT) AA(CT) GA(CT) TC(GATC) AA(CT) GT 128mix

[0059] Primer B: ATG TA(TC) AA(CT) AA(CT) GA(CT) AG(CT) AA(CT) GT 64mix

[0060] Amino acid sequence (45-5...

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Abstract

A method of preparing alpha-1,4-glucan lyase enzymes is described. The nucleic acid sequences coding for the enzymes have also been determined.

Description

technical field [0001] The present invention relates to a method for the preparation of an enzyme, in particular alpha-1,4-glucan lyase ("GL"). The present invention also relates to the nucleotide sequence encoding the enzyme. Background technique [0002] FR-A-2617502 and Baute etc. have reported in Phytochemistry [1988] Vol.27 No.11pp3401-3403 with obvious enzymatic reaction in Common Morchellavulgaris (Morchellavulgaris) to produce 1,5-D-anhydrofructose (" AF"). The yield of AF is rather low. Despite mentioning possible enzymatic reactions, neither document provides any amino acid sequence data, let alone any nucleotide sequence data, for any enzyme. These documents mention that AF can be used as a precursor for the preparation of the antibiotic hydroxymethylpyrone. [0003] Yu et al. in Biochimica et Biophysica Acta [1993] Vol. 1156 pp313-320 reported the preparation of GL from red seaweed and its use for the degradation of α-1,4-glucan to produce AF. The yield of A...

Claims

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Application Information

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IPC IPC(8): C12N15/09C12N1/19C12N1/21C12N9/00C12N9/88C12N15/60C12P19/02C12R1/19C12R1/685C12R1/84C12R1/89C12R1/90
CPCC12P19/02C12N9/88C12N9/00
Inventor 于书坤K·波森K·M·克拉M·波科J·尼尔森J·马库森
Owner DUPONT NUTRITION BIOSCIENCES APS
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