Conotoxin MVII A and Trx fusion protein and its expression and application

A fusion protein and conotoxin technology, applied in the direction of peptide/protein components, DNA/RNA fragments, medical preparations containing active ingredients, etc., can solve the problems of unstable quality, difficult process, low yield, etc., and achieve purification Convenience, simple process, large output effect
CN1487085AInactive Publication Date: 2004-04-07ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2004-04-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention provides one kind of conotoxin MVII A and Trx fusion protein and its expression and application. Trx-CTX MVII A fusion protein is first cloned and expressed in colibacillus and then chromatographicaly purified, reduced and oxidized to obtain active Trx-CTX MVII A fusion protein. The obtained fusion protein may be applied in preparing analgetic for late stage cancer and AIDS and that for post-operation pain, burns, etc. and may be also applied in preparing medicine for cerebral ischemia and cerebral demage owing to its nerve protecting function. The present invention can obtain fusion protein with great molecular weight and relatively long half time, and may be applied in medicine development directly or as intermediate to be enzyme incised to obtain active omiga-conotoxin MVII A for further medicine development.
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Description

technical field

[0001] The invention belongs to genetic engineering and relates to chemically synthesized polypeptides, in particular to the fusion protein of ω-conotoxin MVIIA gene and thioredoxin (Trx) and its expression and application in medicine. Background technique

[0002] Conotoxin (CTX) is a small peptide neurotoxin with specific biological activity secreted by the marine mollusk cone snail. Generally, it contains 10-30 amino acids, and most of them are rich in disulfide bonds. According to their targets and pharmacological activities, they can be divided into α, ω, μ and other families [1] . ω-CTX MVIIA is a voltage sensitive calcium channel (Voltagesensitive calcium channel, VSCC) blocker, which mainly acts on N-type VSCC in nerve tissue. It consists of 25 amino acids, containing 6 Cys residues, forming three pairs of disulfide bonds. Non-clinical studies have proved that it has dual effects. On the one hand, it blocks pain transmission and has an analgesic ef...

Claims

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