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Method for forming block from divided sponge organs

A technology of cell clusters and cells, which is applied in the direction of tissue cell/virus culture devices, tissue culture, biochemical equipment and methods, etc., can solve the problems of the overall growth capacity of cell clusters, and achieve pollution control, small cell damage, The effect of improving the growth ability

Inactive Publication Date: 2004-04-21
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
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Problems solved by technology

Protocells, which are mainly capable of proliferating, are not dominant, which limits the overall growth capacity of the cell mass

Method used

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  • Method for forming block from divided sponge organs
  • Method for forming block from divided sponge organs
  • Method for forming block from divided sponge organs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Material: Membrane sponge, collected in the waters around Dalian.

[0033] Method: Weigh 2.2-2.3 g (wet weight) of two sponges each, and obtain 35 ml of cell suspension by grinding method. The cell density is 8.6×10 as measured by hemocytometer 7 cells / ml.

[0034] The specific operation is as follows:

[0035] 1) Pretreatment: take a fresh sponge, clean the sponge tissue, and remove sand and gravel in the sponge tissue block;

[0036] 2) Cell separation: Cut the washed fresh sponge block into 1-3 mm in calcium and magnesium-free seawater (CMFSW-E) with an EDTA concentration of 10 mmol 3 Grind and squeeze the cell tissue block in a grinding instrument, and squeeze out the cells for 10 minutes, then filter the cell suspension with a 300-500 mesh filter, and obtain a large amount of The discrete cells; the remaining residues were again oscillated in CMFSW-E to be discrete;

[0037] The grinding method described in this embodiment uses a flat-bottomed, plastic circular...

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Abstract

A process for forming the cell aggregate of discrete sponge tissue cells includes such steps as cutting the fresh sponge tissue block in the CaMg-free sea water containing EDTA (CMFSW-E) to become small blocks (1-3 cu.cm), grinding, filtering to obtain the suspension of discrete sponge cells, gradient centrifugation to obtain the discrete cells with high reproduction power, and no microbial pollution, forming aggeregates in aseptic sea water and culturing.

Description

technical field [0001] The present invention relates to the cell in vitro culture technology of marine invertebrate sponge, in particular to the method for forming cell clusters from discrete cells of sponge tissue, which can obtain sponge cell clusters that can be effectively cultured from marine invertebrate sponges. Background technique [0002] Sponges are the most primitive invertebrates in the ocean and belong to the phylum Metazoa. At present, 40% of the natural products found in marine organisms come from sponges [Document 1: Bergmann, W. et al., The isolation of a new thymine pentoside from sponges, . J Am Chem Soc. 1950, 72: 2809- 2810]. These products are not suitable to be obtained by fishing and extraction methods, and are difficult to produce by breeding or synthetic methods. [0003] Taking Halichondrin B. as an example, only 310mg of pure product can be obtained for every ton of sponge (Halichondria okadai) caught. At the same time, fishing for sponge reso...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12N5/00C12N5/06
Inventor 张卫张骁英赵权宇方丽驹
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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