Method for capturing and cultivating caenorhabditis elegans single ovum based on micro-fluidic chip

Abstract

Disclosed is a method for capturing and cultivating a caenorhabditis elegans single ovum based on a micro-fluidic chip. The micro-fluidic chip comprises two layers of polydimethylsiloxane (PDMS). The upper layer is a liquid way layer and is used for capturing, long-time cultivating and observing of the caenorhabditis elegans single ovum. The lower layer is blank PDMS and is connected with the upper PDMS in a sealed mode. The chip captures the caenorhabditis elegans single ovum by a micro-structure and is capable of cultivating the caenorhabditis elegans single ovum alone in a single cultivation room. The method for capturing the caenorhabditis elegans single ovum based on the micro-fluidic chip is easy to operate and the long-time cultivation of the caenorhabditis elegans during a whole life cycle of the caenorhabditis elegans is achieved easily. Exchange of nutrient substance and discharge of metabolic waste are achieved by the chip. The method for capturing the caenorhabditis elegans single ovum based on the micro-fluidic chip facilitates the long-time cultivation of the caenorhabditis elegans and can observe the behaviour and formation of the image of the single caenorhabditis elegans in a long time.

Description

technical field [0001] The invention relates to the field of microfluidics, and in particular provides a method for single egg capture and long-term culture of Caenorhabditis elegans based on a channel microfluidic chip. Background technique [0002] Caenorhabditis elegans is a classic model organism, which is widely used in the fields of modern developmental biology, genetics, genomics and neurobiology. At present, the cultivation of Caenorhabditis elegans is mainly carried out in array microwell plates or agar plates, that is, the nematodes are cultured in 96-well plates or agar plates, and the medium is replaced regularly or the nematodes are transferred to new agar plates. Although the number of nematodes is large, the consumption of reagents and consumables is large; the operation for nematodes is manual, time-consuming and laborious, and the throughput is low; the operation steps are cumbersome and complicated; it is difficult to track and observe the entire life cours...

AI Technical Summary

Problems solved by technology

Although the number of nematodes is large, the consumption of reagents and consumables is large; the operation for nematodes is manual, time-consuming and laborious, and the throughput is low; the operation steps are cumbersome and complicated; it is difficult to track and observe the entire life course of a single nematode

Overall, with the development of nematode-related research, the current development of model biotechnology is far from meeting the needs of modern biomedical research and development

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