Culture pond applied to chip packaging

Abstract

The invention discloses a culture pond applied to chip packaging.The culture pond comprises a big chamber and a small chamber, wherein, the small chamber is arranged inside the big chamber, the culture pond is an inseparable whole. Wherein, the big chamber is provided with a bottom, while the small chamber is a bottomless through chamber, the bottom of the big chamber is completely connected withthe wall of the small chamber; injection molding is carried out on the culture pond as a whole; the external wall of the big chamber of the culture pond is further provided with a groove parallel to the bottom of the big chamber; the bottom of the culture pond is provided with a frame slot matched with the packed chips; the culture pond can also provided with a cover matched with the big chamber and the small chamber.In the invention, the steps for packaging cell chips by the culture pond are simple, and long-term culture of the cell is facilitated, with the culture pond adopted, chip packaging can be finished at only one time.

Description

technical field [0001] The invention relates to a culture pool for chip packaging. Background technique [0002] The culture tank is an indispensable component for cell chip encapsulation. The existing culture tanks for cell chip packaging are roughly divided into two categories: 1. The cavity used by the German MCS (Multi-Channel System) company, etc., adopts a single annular culture tank, and its disadvantage is that the cavity is too large, so that the liquid level is unstable , the flow of the liquid affects the state of the cells, and each culture requires too much medium; 2, the culture chamber designed by Xiang et al. (Xiang G., Pan L., Huang L., et al.Microelectrode array-based system for neuropharmacological applications with cortical neurons cultured in vitro.Biosens.Bioelectron., 22:2478-2484, 2007), they first cut off both ends of the EP tube to obtain a 1cm-long cavity wall, which was glued to the chip surface with PDMS; Then drill a hole with the same diamete...

AI Technical Summary

Problems solved by technology

The existing culture tanks for cell chip packaging are roughly divided into two categories: 1. The cavity used by the German MCS (Multi-Channel System) company, etc., adopts a single annular culture tank, and its disadvantage is that the cavity is too large, so that the liquid level is unstable , the flow of the liquid affects the state of the cells, and each culture requires too much medium; 2, the culture chamber designed by Xiang et al. (Xiang G., Pan L., Huang L., et al.Microelectrode array-based system for neuropharmacological applications with cortical neurons cultured in vitro.Biosens.Bioelectron., 22:2478-2484, 2007), they first cut off both ends of the EP tube to obtain a 1cm-long cavity wall, which was glued to the chip surface with PDMS; Then drill a hole in the center of the 6cm petri dish with the same diameter as the EP tube, set it outside the wall of the EP tube cavity, and fix it with PDMS

Although it avoids the above disadvantages, it is time-consuming and labor-intensive to cut EP tubes and punch holes in the petri dish, the success rate is low, and the smooth cutting surface cannot be guaranteed, which may cause liquid leakage; Part of the chip is suspended and can only be filled with PDMS or epoxy resin, which cannot guarantee level and stability, and excess PDMS or epoxy resin will contaminate the chip

This kind of packaging process is complicated, which is not conducive to mass production

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