Biomolecule motor magnetic regulating and controlling device

A technology of molecular motor and magnetic regulation, applied in nanostructure manufacturing, nanotechnology, nanotechnology, etc., can solve the problems of protein microfilament plus magnetic regulation, which cannot be further promoted and applied, and achieve the effect of a wide range of application prospects

Inactive Publication Date: 2004-07-21
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These characteristics are favored by scientists of nano-electrical machinery systems (NENS), but the above-mentioned protein microfilaments cannot be regulated by external magnetic force, so they cannot be further promoted and applied.

Method used

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  • Biomolecule motor magnetic regulating and controlling device
  • Biomolecule motor magnetic regulating and controlling device
  • Biomolecule motor magnetic regulating and controlling device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0018] Fabrication of molecular motor magnetic control device:

[0019] The base plate of a magnetic control device is connected with a turntable through bearings, and a miniature magnetic control room is arranged on the front surface of the turntable, and the size of the control room is controlled by adjusting screws. Selection, friction wheels (one set of large and one small), and the corresponding fluorescent microscope platform connecting screw, DC micro motor, power supply, molecular motor assembly of inverted fluorescent microscope:

[0020] Pretreatment of metal filaments (20nm diameter): wash with double distilled water for three times, then add small peptides containing His-tag (the small peptides were pre-fluorescent and biotinylated at 50 μM (biotin-PEAC 5 -maleimide) in 1mM PH7.2 Hepes-KOH, 100mM KCl, 2mM MgCl 2 The system reacted at room temperature for 2h), and reacted for 10h. Then buffer A (pH 7.2 10mM Hepes-NaOH / 25mM KCl / 5mM MgCl 2 ) washed five times. Bio...

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Abstract

A nano-class magnetic regulating controller of biomotor features that a controllable magnetic rotor is installed on the platform of microscope, and under the action of external motor, it can rotate fast and drive the molecular motor in specimen platform to rotate.

Description

technical field [0001] The invention belongs to a biological micro-motor system, and in particular relates to an inorganic biological motor and a nano-level biological microelectronic magnetic control device. technical background [0002] Since Japanese scientist Noji published in Nature in 1997 the direct observation of F at the single-molecule level 1 -After the experiment of ATPase rotation, many laboratories in the world have repeated and developed the research results at the single-molecule level under different conditions and environments. The rapid rotation of a protein filament fluorescent molecule connected to its F1-ATPγ subunit can be observed by single-molecule technology during the hydrolysis of ATPase: when an ATP molecule is hydrolyzed, it can produce fluorescently labeled protein filaments (actin ) 120o rotation. It is well known that studying F at the single-molecule level 1 - In addition to the important scientific significance of the structure and funct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B82B3/00
Inventor 乐加昌聂棱张春野张英豪王兴胜崔元波
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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