Culture media for leukemia cell inducel production of dendrite cell and its culturing method and application

Abstract

A culture medium for inducing the leukemia cell to generate dendric cell (DC) is prepared through adding the angelica polyose to the convertional non-serum culture medium of leukemia cell for promoting the function of hematopoietic cell, immunity and the phagocytic function of mononuclear-macrophage, and inducing the differentiation of leukamia cell strain K562. Its advantages are high molecular expression of its antigen to costimulation, and short preparing time (3-5 days).

Description

technical field [0001] The invention relates to a culture medium for cells, a culture method and application thereof, in particular to a culture medium for inducing dendritic cells from leukemia cells, a culture method and application thereof. Background technique [0002] Dendritic cells (DC) are the most important antigen-presenting cells that initiate the initial response of T cells, and DC can be used for tumor immunotherapy. Traditional DC preparations use bone marrow hematopoietic progenitor cells and peripheral blood mononuclear cells. The preparation method is to add granule-macrophage colony-stimulating factor (GM-CSF), interleukin 4 (IL-4) and tumor necrosis factor (TNF) into a serum culture system. In addition, in the preparation process, it is necessary to add specific antigens extracted from tumor cells for shock culture, the process is complicated, and the culture time is long, about 15 days. In recent years, DCs have also been prepared from leukemia cells. T...

AI Technical Summary

Problems solved by technology

But the disadvantage is that the traditional method is used to prepare leukemia DC, the expression of antigenic co-stimulatory molecules is low, the effect of activating T cells is poor, and the culture time is still long, about 13 days