Check patentability & draft patents in minutes with Patsnap Eureka AI!

Method for preparing leather using protease and method for treating wastes dervied from leather processing using same

A technology of protease and leather, which is applied in small raw hide/big raw hide/leather/fur treatment, small raw hide/big raw hide/leather skin/fur chemical treatment, tanning treatment, etc. It can solve the problem of increasing production cost by processing cost

Inactive Publication Date: 2004-09-15
INSECT BIOTECH
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cost of disposal of this solid waste increases the cost of production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing leather using protease and method for treating wastes dervied from leather processing using same
  • Method for preparing leather using protease and method for treating wastes dervied from leather processing using same
  • Method for preparing leather using protease and method for treating wastes dervied from leather processing using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: Production protease HY-3

[0043] For the production of protease HY-3, the standard medium used for culturing microorganisms was sterilized at 121° C. for 20 minutes under high pressure, and then Aranicola proteolyticus HY, which produces protease HY-3, was added in an amount of 0.1-5% by volume based on the total volume of the medium -3 strain (KCTC 0268BP) and cultivated at 25-30°C for 25-30 hours. The medium is subjected to membrane filtration, which separates the supernatant from the biomass. When necessary, the supernatant was concentrated 3-10 fold by filtration through a 10 kDa membrane.

Embodiment 2

[0044] Example 2: Analysis of proteins eluted from liming and deliming steps using protease HY-3

[0045] In order to study the role of protease HY-3 in the liming and deliming steps which greatly affect COD, the following procedure was carried out.

[0046] Specifically, 20 g of raw hide was cut into an appropriate size, put into a 50 ml test tube, and then 10 ml of water was added thereto. Thereafter, 0.5% sodium bisulfite and 0.5% ammonium sulfate were added and reacted at room temperature for 15 minutes. After the reaction, 0.2% detergent and 0.5% degreaser were added to the resulting solution and reacted at room temperature for 30 minutes. Three tubes were divided into a control containing no protease HY-3, and containing the supernatant prepared as in Example 1 above and the concentrated supernatant. Allow each tube to stand at room temperature and obtain a sample. The protein analysis kit (Bio-rad, USA) modified from the Bradford method (Bradford, M., Anal. Biochem.,...

Embodiment 3

[0048] Example 3: Use of protease HY-3 in the softening step

[0049] In order to improve the stability and performance of the protease HY-3 in leather processing, the protease was formulated and thus used in the softening step.

[0050] The lyophilized protease HY-3 of the present invention is added to 40-50% ammonium chloride (NH 4 Cl), 40-50% ammonium sulfate ((NH 4 ) 2 SO 4 ), 0.005-0.01% calcium chloride (CaCl 3 ) and 0.025-0.1% lactose. In order to examine the softening effect of the formulated protease HY-3, hides were subjected to a deliming step followed by a softening step using different enzyme preparations, after which the surface state of the hides was observed. In addition, hides and skins are subjected to tanning and finishing steps, and then the surface state is observed. As protease preparations used in the softening step for comparison, Amoron (Chungmu Fermentation, Korea) and Oropon K (TFL, Germany) were used. The experimental procedure is summarized ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Login to View More

Abstract

The present invention relates to a method for preparing leather using a protease and a method for treating wastes derived from leather processing using the same, which are advantageous in terms of preparation of leather of excellent quality, decreased waste production by reducing the amount of chemicals, and treatment or recycling of wastes in an environmentally friendly manner. The protease HY-3 produced from Aranicola proteolyticus HY-3 strain is added to the steps of soaking, liming, deliming and bating, among leather processing, and thus the leather is prepared. Additionally, the protease HY-3 is added to wastewater and solid wastes generated during the soaking and liming steps, and solid wastes generated during the liming, deliming, bating and finishing steps, so that the wastes are treated.

Description

technical field [0001] The present invention relates to a process for the preparation of leather using protease HY-3 and a process for using it to treat waste from leather processing. More specifically, the present invention relates to a method for preparing leather, wherein protease HY-3 produced from Aranicola proteolyticus HY-3 strain is added to the soaking, liming, deliming and softening steps; Process for waste water and solid waste from soaking and liming steps, and solid waste from liming, deliming, softening and finishing steps, which has the advantage of producing leather of superior quality and environmentally friendly treatment or recycling of waste . Background technique [0002] In general, the leather industry is a chief industry that determines the quality of leather products such as shoes, bags, garments, and belts as a rough leather processing industry requiring expensive equipment and technology. In the leather industry, when hides or hides of various an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C14C1/06C14C1/08
CPCC14C1/065C14C1/08
Inventor 朴镐用孙光熙权容国申东夏闵升基
Owner INSECT BIOTECH
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com