Method for determining sensitivity of limulus reagent

A technology of sensitivity and limulus reagent, which is applied in the detection field of bacterial endotoxin, can solve the problems of easy human error, long test preparation time, and long reaction time, and achieve objective results, short test preparation time, and short reaction time. Effect

Inactive Publication Date: 2004-10-13
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

[0005] Whether it is qualitative or quantitative determination of the content of endotoxin by the limulus test, the accuracy of the sensitivity of the limulus reagent is crucial; the conventional method of measuring the sensitivity of the limulus reagent is the limited method: the pre-determination and the formal determination are carried out respectively. The bacterial endotoxin working

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  • Method for determining sensitivity of limulus reagent
  • Method for determining sensitivity of limulus reagent

Examples

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Embodiment 1

[0018] 1) Test of single concentration standard endotoxin on different Limulus reagents

[0019] Adopt standard endotoxin concentration 30EU / ml, carry out three repeated experiments to the standard limulus reagent that sensitivity is 0.01EU / ml respectively, obtain the mean value and standard deviation of corresponding reaction time T (OD0.02) (1) 350 ± 80 ( Second);

[0020] Using the standard endotoxin concentration of 4.4EU / ml, the standard limulus reagent with a sensitivity of 0.06EU / ml was tested five times to obtain the mean value and standard deviation of the corresponding reaction time T (OD0.02) (2) 775± 50(seconds);

[0021] Using the standard endotoxin concentration of 2.2EU / ml, three repeated tests were carried out on the standard LAL reagent with a sensitivity of 0.125EU / ml, and the mean value and standard deviation of the corresponding reaction time T (OD0.02) were obtained (3) 1035 ± 120 (Second);

[0022] Using the standard endotoxin concentration of 4.4E...

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Abstract

The invention relates to the detection for bacterial endotoxin, concretely a limuloid reagent sensitivity determining method: 1) adopt standard endotoxin at the same concentration to repetitively test standard limuloid reagents at different sensitivities on the endotoxin determiner, to obtain a average value and standard difference of the corresponding characteristic reacting time; 2) make the limuloid reagent to be measured react with the above standard endotoxin to obtain a corresponding characteristic reacting time, then compare the characteristic reacting time whit the above average value and standard difference to judge and obtain the limuloid reagent sensitivity.

Description

technical field [0001] The invention relates to the detection of bacterial endotoxin, in particular to a method for measuring the sensitivity of Limulus reagent. Background technique [0002] Bacterial endotoxin (Bacterial Endotoxin) is a macromolecular substance with various biological activities produced by Gram-negative bacteria, and its main chemical component is lipopolysaccharide (LPS); endotoxin is the main pollution in injection medicine (raw materials, etc.) substance. [0003] The detection methods of bacterial endotoxin include rabbit test method and limulus test method. Limulus Amebocyte Lysate (LAL, or Tachypleus Amebocyte Lysate, TAL) is an in vitro detection method that uses the mechanism of agglutination reaction between Limulus Amebocyte Lysate and endotoxin to qualitatively or quantitatively detect drug or bacterial endotoxin in the body's blood. . Use chromogenic or fluorescent peptide compounds for the analysis of endotoxins, compound structure R1-A1-A...

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Application Information

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IPC IPC(8): G01N33/50G01N33/52G01N33/579
Inventor 李京华邵英光王俊德
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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