Method for preparing multifunctional biological repair material

A bioremediation and multifunctional technology, applied in the field of biomedicine, achieves the effects of low price, adjustable degradation speed and convenient use

Inactive Publication Date: 2004-10-27
刘永庆
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chitosan, known as nerve repair, still has some problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0017] Example 1:

[0018] (1) Dissolve chitosan with a degree of deacetylation of 50% and a molecular weight of 400,000 daltons, type I collagen, and sodium hyaluronate in 1% acetic acid solution or distilled water to prepare a shell with a concentration of 2% Glycan acetic acid solution, 1% type I collagen acetic acid solution and 1% sodium hyaluronate aqueous solution; adjust the pH of the sodium hyaluronate aqueous solution to about 3 with 1M HCI, and then press 1:50:200 (v / v) Mix the sodium hyaluronate solution with the collagen and chitosan solution evenly.

[0019] (2) Put the prepared solution into a container and freeze-dry to form a sponge layer film with a thickness of 3 mm or a porous tubular material with an inner diameter of 2 mm and an outer diameter of 5 mm; (Note: The tubular material is chitosan / collagen / Sodium hyaluronate solution is injected into a circular container made of concentric tubular polytetrafluoroethylene, and then quickly frozen and dried.)

[00...

Example Embodiment

[0022] Example 2:

[0023] (1) Dissolve sodium hyaluronate, type II collagen, chitosan with a degree of deacetylation of 70% and a molecular weight of 200,000 daltons in distilled water or 1% acetic acid solution to make a transparent solution with a concentration of 10% Sodium hydrochloride aqueous solution, 2% type I collagen, 1% chitosan acetic acid solution; adjust the pH of the sodium hyaluronate aqueous solution to about 2 with 1M HCI, and then press 1:20:50 (v / v) Mix the sodium hyaluronate solution with the collagen and chitosan solution evenly.

[0024] (2) Put the prepared solution into a container and freeze-dry to form a sponge with a thickness of 4 mm or a porous tubular material with an inner diameter of 3 mm, an outer diameter of 5 mm, and a length of 15 mm;

[0025] (3) Soak the sample with 40% ammonia water for 10 minutes; then rinse it with distilled water and freeze-dry it.

[0026] (4) Apply a thin layer of 2% chitosan solution (bake it until it stops dry, apply...

Example Embodiment

[0028] Example 3:

[0029] (1) Dissolve sodium hyaluronate, type IV collagen, chitosan with a degree of deacetylation of 90% and a molecular weight of 10,000 Daltons in distilled water or 2% acetic acid solution to prepare a chitosan with a concentration of 3%. Sugar solution, 0.1% sodium hyaluronate aqueous solution, 2% type I collagen, 2% chitosan acetic acid solution; adjust the pH of the sodium hyaluronate aqueous solution to about 1 with 1M HCI, and then press 1:1 :1 (v / v) ratio, mix the sodium hyaluronate solution with collagen and chitosan solution evenly.

[0030] (2) Put the prepared solution into a container and freeze-dry to form a sponge with a thickness of 0.5 mm or a porous tubular material with an inner diameter of 2 mm and an outer diameter of 3 mm;

[0031] (3) Soak the sample in 25% ammonia water for 20 minutes; then rinse it with distilled water, and then freeze-dry it.

[0032] (4) When the solution prepared in step (1) is dried until it is not dry, add a trace...

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Abstract

A multifunctional biologic material for repairing skin, nerve and cartilage is prepared from chitosan, collagen and sodium hyaluronate through preparing their solutions, proportionally mixing, defomaing, loading in mould, freeze drying to become film or tube, immersing in ammonia water, drying, coating a solution layer of chitosan, chitosan/sodium hyaluronate, chitosan/collagen, or chitosan/collagen/sodium hyaluronate, and drying. Its advantages are high biocompatibility, mechanical strength and antibacterial power.

Description

technical field [0001] The invention relates to a preparation method of biological materials used for skin, nerve and cartilage repair, which mainly uses chitosan / collagen / sodium hyaluronate as raw materials to prepare thin film or tubular materials, and belongs to the technical field of biomedicine. Background technique [0002] The skin and nervous system are the most important tissues and organs in the human body. Once damaged, their normal functions will be affected. Although the regeneration ability of the skin is very strong, it is far from enough to heal large area burns or wounds by self-skin or autograft skin alone, and it is necessary (artificial skin as) a temporary wound surface during the treatment process Protective covering materials come to help. Artificial skin made of synthetic materials or collagen has been used clinically for many years, but there are certain defects. Chitosan is the only basic polysaccharide among the known natural polysaccharides. Its...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/60
Inventor 刘永庆
Owner 刘永庆
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