Use of pilose asiabell and astragalus composition in preventing and treatnig acute pulmonary hurt
A technique for acute lung injury and composition, applied in the application field of Codonopsis astragalus composition in prevention and treatment of acute lung injury
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[0022] Codonopsis pilosula and Astragalus membranaceus are removed from impurities and processed into decoction pieces. Accurately weigh 400 grams each of Dangshen Radix Astragali, rinse with deionized water, add 3200ml of deionized water, heat and extract for 1 hour, and release the extract; then add 2400ml of deionized water, heat and extract for 1 hour, and then release the extract; Add 2400ml of deionized water, heat and extract for 0.5 hours, and release the extract. Merge after three extracts are filtered, concentrate to 600ml, then add the ethanol that concentration is 95% to make alcohol content be 60%, precipitate for 24 hours, filter; 80%, precipitated, filtered, recovered ethanol and concentrated to 400g to obtain the Codonopsis and Radix Astragali extract composition.
[0023] The medicine of the present invention can be stored in cold storage with an appropriate amount of ethanol, or stored at room temperature after being dried. In the medicine of the present in...
experiment example
[0025] 1 experimental drug
[0026] Codonopsis pilosula and Radix Astragali extract composition: Codonopsis pilosula and Radix Astragali extract composition prepared according to the above examples, wherein every 20ml contains 3.25g of the same amount of Codonopsis pilosula and Radix Astragali extract. During the experiment, it was concentrated in a water bath from 2ml to 1ml, and sterilized by circulating steam for 40 minutes Sterilize.
[0027] LPS: (US Sigma Company, powder, diluted with sterile saline before use).
[0028] Dexamethasone: (Chengdu First Pharmaceutical Factory)
[0029] 2 Experimental animals: Wistar rats (provided by the Animal Center of West China Campus, Sichuan University, second-level microbiological control level)
[0030] 3 Experimental methods:
[0031] group :
[0032] 60 rats were randomly divided into normal control group, modeling group (LPS+NS group), dexamethasone intervention (treatment) group (LPS+DEX group) and Shenqi Fuzheng injection ...
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