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Vesicles derived from T cells, production and uses

A technology of immunogenicity and composition, applied in the direction of cell culture active agents, animal cells, vertebrate cells, etc., can solve the problems of biological function description of T cell exosomes, effective methods for vesicles without biological activity, etc.

Inactive Publication Date: 2005-07-13
阿诺塞斯公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, until now, the biological functions of these T cell exosomes have not been described, and there have been no reports of efficient methods for preparing these vesicles in a bioactive state.

Method used

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  • Vesicles derived from T cells, production and uses
  • Vesicles derived from T cells, production and uses
  • Vesicles derived from T cells, production and uses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0136] Materials and Methods

[0137] 1. Vesicle Generation from Primary T Cells and T Cell Lines

[0138] 1.1 Vesicle generation from primary T cells

[0139] CD3+ T cells were enriched and purified to 90% from unadsorbed PBMC cells by removing non-T-cells with nylon fiber columns. Enriched CD3+ T cells were diluted to 4-5×10 6 / ml and cultivated in filtered AIMV medium by one of the following methods:

[0140] a. 1μl / ml PHA culture for 3 days

[0141] b. 5μl / ml PMA plus 250ng / ml ionomycin cultured for 3 days

[0142] c. Culture with 1 μl / ml PHA for 2 days. After replacing with fresh and filtered AIMV medium, culture was continued for another 4 days.

[0143] 1.2 Generation of vesicles from a T cell line (Jurkat)

[0144] Jukat cells were diluted to 4-5 x 10 6 / ml, and cultured in AIMV medium with filtered PHA concentration of 1 μl / ml for 4 days.

[0145] 1.3 Purification of T cell-derived vesicles

[0146] The resulting vesicles were purified from T cell culture su...

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Abstract

The present invention relates to a composition comprising vesicles released from activated T lymphocytes, and methods for its production and application. The vesicles contain a series of biologically active molecules that confer upon them notable properties such as: antigen recognition, antigen presentation, and other regulatory and effector functions. The present invention also relates to the transfer or delivery of antigenic molecules (such as peptides, peptide / MHC complexes, TCR and its subunits, etc.) using the above-mentioned vesicles to antigen-presenting cells (APCs) to induce specific immune responses, especially specific CTLs method of answering. The present invention also relates to methods for selectively or specifically delivering molecules to target cells using the above-mentioned vesicles.

Description

[0001] Introduction [0002] The present invention relates to a composition comprising vesicles released from activated T lymphocytes, and methods for its production and application. The vesicles contain a series of biologically active molecules that confer upon them notable properties such as: antigen recognition, antigen presentation, and other regulatory and effector functions. The present invention also relates to the transfer or delivery of antigenic molecules (such as peptides, peptide / MHC complexes, TCR and its subunits, etc.) Method of CTL response. The present invention also relates to methods for selectively or specifically delivering molecules to target cells using the above-mentioned vesicles. Background of the invention [0003] The immune system consists of two basic components: leukocytes and soluble mediators. Different leukocyte subsets constitute an immunoregulatory network in which the maturation and activation of each class is influenced by the others. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/02A61K35/26A61K39/00A61K39/29A61K39/39A61K45/00C07K14/18C12N5/0783C12N5/0784C12N5/10
CPCA61K39/29A61K2039/5154A61K2039/5158A61K2039/55555C07K14/005C12N5/0636C12N5/0639C12N2500/14C12N2501/20C12N2501/515C12N2510/00C12N2510/02C12N2770/24222C12N2770/24234A61K39/12
Inventor 侯亚非徐迪惠阿妮塔·梅赫塔-达马尼亨利·兰帕斯基佩德罗·帕斯吉恩-伯纳德·勒佩克
Owner 阿诺塞斯公司
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