Microscopic automatic focusing device and method suitable for space application

An automatic focusing and spatial technology, applied in microscopes, image data processing, optics, etc., can solve the problems that the container wall interference cannot be eliminated, it is not suitable for automatic focusing, and the precision is not high. focus precise effect

Inactive Publication Date: 2005-07-20
SHANGHAI INST OF TECHNICAL PHYSICS - CHINESE ACAD OF SCI
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Problems solved by technology

The shortcoming of this ranging automatic focusing method is that the accuracy is not high, and the interference of the container wall cannot be eliminated, so it is not suitable for microscopic automatic focusing in space applications
Others, such as the Shenzhou-2 space crystal observation device, belong to macroscopic observation. It uses the spectrum method for automatic focusing. Not suitable for automatic focusing of microscopic targets with spatial distribution

Method used

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  • Microscopic automatic focusing device and method suitable for space application
  • Microscopic automatic focusing device and method suitable for space application
  • Microscopic automatic focusing device and method suitable for space application

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Embodiment Construction

[0038] A preferred embodiment of the present invention is given below according to Fig. 1 to Fig. 6, and the present invention is further described in detail in combination with this embodiment.

[0039] Please refer to FIG. 1 , this embodiment includes a microscopic optical system 40 , an image acquisition system 10 , an image processing system 20 and a micro-displacement control system 30 . Wherein, in the image acquisition system 10 , the CCD imaging device 11 is installed on the imaging surface of the microscopic optical system 40 to acquire the microscopic image of the target. The microscopic image is passed through the image digitization module 12 to obtain a digital microscopic image, and stored in the image buffer module 13 . The image processing system 20 reads the image in the image buffer module 13 to the internal RAM through the DSP interface circuit 21 in DMA mode, and then performs focusing processing through the DSP processor 22 to obtain a clear imaging positio...

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Abstract

This is a kind of microscopical focus self-adjusting device and method fit for spatial application. The device comprises the microscopical optical system which orderly compose optical couplings with dimensional objects, the system to obtain microscopical images, the image processing system which is used to process the microscopical images to ascertain the clear imaging position, the coupling image processing system, and the micro-displacement controlling system of the microscopical optical system, so as to form a closed loop controlling system and realize microscopical focus self-adjusting. The method is to obtain the microscopical images mainly depending on the micro-displacement controlling system to make the microscopical optical system move the distance with spatial objectives step by step in stated bound. Then calculate every image element in the way of gray difference and work out the gray maximum after doing a group of gray extremum. Ascertain the clear imaging position by the gray maximum and adjust the distance between the microscopical optical system and the spatial objectives by the micro-displacement system to realize focus self-adjusting.

Description

Technical field: [0001] The invention relates to a space microscopic auto-focusing device and method, in particular to a microscopic auto-focusing device and method for spatially three-dimensionally distributed cell targets suitable for space applications. Background technique: [0002] Space life science is a brand-new field that is actively researched by countries all over the world. Microscopic observation and research in space is of great significance to the development of space life science. The object of spatial microscopic observation is the three-dimensional distribution of cells in an airtight container, which is very different from the object of ground microscopic observation on slides. Due to the effect of gravity on the ground, the target is flattened into a layer, which can be regarded as a plane; in space, the gravity almost disappears, and the cells are suspended in the container, showing a three-dimensional distribution in space. To perform microscopic obser...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/32G02B21/36G06T1/00
Inventor 郑伟波张涛刘学明吕银环卢晋人童广辉
Owner SHANGHAI INST OF TECHNICAL PHYSICS - CHINESE ACAD OF SCI
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