Purple laver protein and polysaccharide product, and method of ultrasonic assistant extraction

An ultrasonic-assisted extraction and ultrasonic-assisted technology, which is applied to the preparation method of peptides, chemical instruments and methods, plant peptides, etc., can solve the problems of no laver protein and polysaccharide products, long extraction time, protein loss, etc.

Inactive Publication Date: 2005-10-26
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the research on the extraction of laver polysaccharides, the laver protein is removed as an impurity, and the method of removing protein often requires the method of solvent precipitation for many times, which will cause protein denaturation and pollution, resulting in the content of laver as high as 40 % protein loss
All in all, traditional polysaccharide or protein extraction methods generally have problems such as long extraction time, l...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Weigh 1000g of dried laver, crush it to 40 mesh, and use supercritical CO 2 Extraction technology Degreasing treatment, the extraction temperature is 35°C, the extraction pressure is 22MPa, and the extraction time is 4h; weigh 500g of defatted laver powder, add 50 times of tap water to dissolve, and heat the material liquid to 30°C for ultrasonic-assisted laver protein and polysaccharide extract. Ultrasonic-assisted extraction is carried out by applying ultrasonic waves intermittently. The extraction conditions are: extraction time 40min, ultrasonic pulse width 5s, pulse interval 2s, the material liquid passes through the ultrasonic probe in a circular manner, and the ultrasonic power is 1kW. The obtained extract The protein content is 44.2% (dry basis), the polysaccharide content is 26.5% (dry basis), the extract is filtered, concentrated 20 times, and further formulated into laver protein / polysaccharide oral liquid, or it is spray-dried to obtain laver protein / polysac...

Embodiment 2

[0015] Weigh 800g of dried seaweed, crush it to 40 mesh, and use supercritical CO 2 Extraction technology Degreasing treatment, the extraction temperature is 50°C, the extraction pressure is 35MPa, and the extraction time is 2h. Weigh 500g of defatted laver powder, add 80 times of tap water to dissolve, and heat the feed solution to 50°C for ultrasonic-assisted extraction of laver protein and polysaccharide. Ultrasonic-assisted extraction is carried out by applying ultrasonic waves intermittently. The extraction conditions are as follows: extraction time 70 minutes, ultrasonic pulse width 3 s, pulse interval 1 s, the material liquid passes through the ultrasonic probe in a circular manner, and the ultrasonic power is 1.5 kW. The protein content in the obtained extract is 38%, and the polysaccharide content is 31%. The extract is filtered, concentrated 30 times, and further spray-dried to obtain laver protein / polysaccharide powder, wherein 42.3% of laver protein and 28.1% of la...

Embodiment 3

[0017] Weigh 5000g of fresh seaweed, beat it, add tap water 1 times the weight of seaweed pulp to the beaten seaweed, and use ultrasonic-assisted extraction technology to extract active ingredients such as protein and polysaccharides. The extraction conditions are: material liquid temperature 25 °C, The extraction time is 60min, the ultrasonic pulse width is 8s, and the pulse interval is 6s. The laver water extract is centrifugally filtered with a 100-mesh filter cloth, then concentrated to a solid content of 40%, and spray-dried to obtain laver protein / polysaccharide powder. Protein 48.2%, laver polysaccharide 22.4%.

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Abstract

The present invention provides a porphyrilic protein, polysaccharide product and its ultrasonic auxiliary extraction method. Its technological process includes the following steps: pulverizing, defatting and ultrasonic auxiliary water extracting dried porphyra, filtering, concentrating, spray-drying porphyra water extract to obtain porphyrilic protein and polysaccharide powder. The obtained product contains 40-50% of protein and 22-30% of polysaccharide compount, and said porphyrilic protein and porphyrilic polysaccharide have the functions of raising immunity, regulating blood fat, resisting senility and resisting virus, etc.

Description

Technical field: [0001] The invention relates to the field of development and utilization of marine plant products, in particular to the production of health food, in particular to a laver protein and polysaccharide product and an ultrasonic-assisted extraction method thereof. Background technique: [0002] Seaweed is rich in proteins, polypeptides, amino acids, nucleic acids, sugars, fatty acids, vitamins, inorganic elements, etc., and its nutritional value is very rich. [0003] my country is one of the three laver producing countries in the world, after Japan and South Korea. Porphyra has a high development and utilization value, but the current laver processing is mainly laver laver, and a small number of it is added to other foods after being crushed. On the whole, the level of laver processing is relatively low, the depth of product development is not enough, the variety is single, and it lacks competitiveness. In recent years, there have been some achievements in th...

Claims

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Application Information

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IPC IPC(8): C07K14/415
Inventor 马海乐
Owner JIANGSU UNIV
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