Purple laver protein and polysaccharide product, and method of ultrasonic assistant extraction
An ultrasonic-assisted extraction and ultrasonic-assisted technology, which is applied to the preparation method of peptides, chemical instruments and methods, plant peptides, etc., can solve the problems of no laver protein and polysaccharide products, long extraction time, protein loss, etc.
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Embodiment 1
[0013] Weigh 1000g of dried laver, crush it to 40 mesh, and use supercritical CO 2 Extraction technology Degreasing treatment, the extraction temperature is 35°C, the extraction pressure is 22MPa, and the extraction time is 4h; weigh 500g of defatted laver powder, add 50 times of tap water to dissolve, and heat the material liquid to 30°C for ultrasonic-assisted laver protein and polysaccharide extract. Ultrasonic-assisted extraction is carried out by applying ultrasonic waves intermittently. The extraction conditions are: extraction time 40min, ultrasonic pulse width 5s, pulse interval 2s, the material liquid passes through the ultrasonic probe in a circular manner, and the ultrasonic power is 1kW. The obtained extract The protein content is 44.2% (dry basis), the polysaccharide content is 26.5% (dry basis), the extract is filtered, concentrated 20 times, and further formulated into laver protein / polysaccharide oral liquid, or it is spray-dried to obtain laver protein / polysac...
Embodiment 2
[0015] Weigh 800g of dried seaweed, crush it to 40 mesh, and use supercritical CO 2 Extraction technology Degreasing treatment, the extraction temperature is 50°C, the extraction pressure is 35MPa, and the extraction time is 2h. Weigh 500g of defatted laver powder, add 80 times of tap water to dissolve, and heat the feed solution to 50°C for ultrasonic-assisted extraction of laver protein and polysaccharide. Ultrasonic-assisted extraction is carried out by applying ultrasonic waves intermittently. The extraction conditions are as follows: extraction time 70 minutes, ultrasonic pulse width 3 s, pulse interval 1 s, the material liquid passes through the ultrasonic probe in a circular manner, and the ultrasonic power is 1.5 kW. The protein content in the obtained extract is 38%, and the polysaccharide content is 31%. The extract is filtered, concentrated 30 times, and further spray-dried to obtain laver protein / polysaccharide powder, wherein 42.3% of laver protein and 28.1% of la...
Embodiment 3
[0017] Weigh 5000g of fresh seaweed, beat it, add tap water 1 times the weight of seaweed pulp to the beaten seaweed, and use ultrasonic-assisted extraction technology to extract active ingredients such as protein and polysaccharides. The extraction conditions are: material liquid temperature 25 °C, The extraction time is 60min, the ultrasonic pulse width is 8s, and the pulse interval is 6s. The laver water extract is centrifugally filtered with a 100-mesh filter cloth, then concentrated to a solid content of 40%, and spray-dried to obtain laver protein / polysaccharide powder. Protein 48.2%, laver polysaccharide 22.4%.
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