Mutagenesis method of fungus for preparing Tacrolimus

A technology of tacrolimus and bacterial strains, applied in biochemical equipment and methods, bacteria, treatment of microorganisms with electricity/wave energy, etc., can solve unseen problems, achieve simple equipment, safe operation, and great promotion value Effect

Inactive Publication Date: 2005-10-26
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no relevant patents and literature reports on the application of laser in the breeding of tacrolimus strains

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] At room temperature, take slant made with sterile water for 10 5 ~10 7 5mL of the spore / mL suspension was placed in a sterile small test tube with a diameter of 2cm, irradiated with a He-Ne laser (λ=632.8nm) for 5min at a power of 10mW, and the treated suspension was spread on a flat plate. The positive variation rate of the mutant strain was 6-8%. The fermentation unit is increased by 12-13%.

Embodiment 2

[0012] At room temperature, take inclined spores and make 10 with sterile water 5 ~10 7 spores / mL suspension, take 5 mL of the suspension irradiated by He-Ne laser and place it in a sterile small test tube with a diameter of 2 cm to irradiate with He-Ne laser (λ=632.8nm) for 5 min, after the irradiation power is 20 mW. The suspension was spread on the plate. The positive variation rate of the mutant strain was 12-13%. The fermentation unit increased by 17-18%.

Embodiment 3

[0014] At room temperature, take inclined spores and make 10 with sterile water 5 ~10 7 spores / mL suspension, take 5 mL of the suspension irradiated by He-Ne laser and place it in a sterile small test tube with a diameter of 2 cm to irradiate with He-Ne laser (λ=632.8nm) for 15 min, after the irradiation power is 20 mW. The suspension was spread on the plate. The positive variation rate of the mutant strain was 22-23%. The fermentation unit increased by 27-28%.

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Abstract

The invention discloses a fungus mutagenesis method to produce, Tacrolimus. The method includes the process as follow: in the room temperature, make bevel spore into spore suspended liquid by asepsis water and enclose them into asepsis cuvette, let them be radiated in some time by He-Ne,then dilute it and besmear it to No1 cultivating basis flat to get seduced streptomycin with jarless heredity. This method has simple equipment, easily doable and safe operation.

Description

technical field [0001] The invention relates to a strain mutagenesis method for preparing tacrolimus, which belongs to the strain mutation breeding technology. Background technique [0002] Since the 1990s, my country's organ transplant work has leapt to the top in Asia. There are more than 100 hospitals and more than 200 experts nationwide who can perform 25 kinds of allogeneic organ transplants. Immunosuppressants are essential drugs for organ transplantation. The application of immunosuppressants such as cyclosporine is limited due to its nephrotoxicity, and the demand for new immunosuppressants is urgent. Many new immunosuppressants have been successfully marketed in my country, among which FK506 (Tacrolimus) has the fastest growth rate. The Chinese name of FK506 is Tacrolimus, and its trade name is Prograf. Compared with cyclosporine, tacrolimus has the following characteristics: ① strong immunosuppressive effect; ② less acute and chronic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 闻建平卢文玉冯伟财音青格乐
Owner TIANJIN UNIV
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