Process for producing theanine

一种制造方法、茶氨酸的技术，应用在制造茶氨酸领域，能够解决茶氨酸精制麻烦等问题，达到有利于工业生产、高效制造、生产简单的效果

Inactive Publication Date: 2005-10-26
TAIYO KAGAKU CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has the problem that glutamic acid is also produced as a by-product along with the production of theanine by the hydrolysis reaction of glutaminase, which makes the purification of theanine troublesome.

Method used

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  • Process for producing theanine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Isolation of Theanine Nutritive Bacteria (utilizing bacteria)

[0018] The soil from Shiga Prefecture and Kyoto Prefecture in Japan was used to prepare a soil suspension, and a selective medium with theanine as a carbon source was used, in which 0.5% theanine, 0.03% yeast extract, and KH 2 PO 4 0.05%, K 2 HPO 4 0.05%, MgSO 4 ·7H 2 O 0.03%, pH 7, subcultured for 3 times, and isolated 100 strains of theanine-trophic bacteria.

Embodiment 2

[0019] The preparation of embodiment 2 cell-free extract

[0020] In 1 liter of the selection medium of Example 1, 100 strains of theanine-trophic bacteria were respectively cultured at 30° C. for 20 hours. Then the bacterial cells were collected and washed, suspended in 50 ml of 30 mM potassium phosphate buffer (pH 7.0), and ultrasonically disrupted at 5° C. to 20° C. to obtain a cell-free extract.

Embodiment 3

[0021] Embodiment 3 enzyme reaction

[0022] Use the cell-free extract of embodiment 2, in the 100mM boric acid buffer (Na 2 B 4 o 7 -NaOH, pH 11), theanine was synthesized by performing an enzyme reaction at 30°C for 24 hours.

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Abstract

It is intended to provide a novel process for efficiently producing theanine, thereby enabling convenient and industrially advantageous production of theanine. Pseudomonas citronellosis GEA, which has been newly isolated from soil in nature and selected, is a theanine-producing strain belonging to the species citronellosis of the genus Pseudomonas and showing a gamma-glutamyl transfer reaction. Theanine can be highly efficiently produced, compared with the existing methods, by using glutaminase originating in the above strain in a mixture of glutamine with ethylamine at pH 9 to 12.

Description

technical field [0001] The present invention relates to a new method of manufacturing theanine Background technique [0002] Theanine is a known main component of the taste of green tea, and is an important flavoring substance of foods represented by tea. In addition, it has been pointed out in literature that γ-glutamine derivatives including theanine act as physiologically active substances in animals and plants. For example, in Chem.Parm.Bull., 19(7), 1301-1307 (1971); 19(6), 1257-1261 (1971) in the same publication; 34(7), 3053-3057 in the same publication (1986); Journal of Pharmaceutical Sciences, 95 (7), 892-895 (1975) and other documents, revealing that theanine, glutamine, etc. can inhibit convulsions caused by caffeine. This shows that these compounds have effects on the central nervous system, and are expected to function as effective physiologically active substances. [0003] Theanine has been commonly manufactured by extracting it from dried tea leaves obtai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/02C12P13/04C12R1/38
CPCC12P13/04C12P13/02
Inventor 立木隆冈田幸隆小关诚大久保勉L·R·朱内贾山崎长宏
Owner TAIYO KAGAKU CO LTD
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