Technique for preparing malonyl ginsenoside, and application of medication in treating diabetes

A technology of malonyl ginsenosides and ginsenosides, which can be used in drug combinations, medical preparations containing active ingredients, metabolic diseases, etc., and can solve problems such as the loss of water-soluble saponins.

Inactive Publication Date: 2005-11-16
JILIN AGRICULTURAL UNIV
View PDF0 Cites 17 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, in the commercial production of malonyl ginsenosides, when the total saponins are extracted with n-butanol, these saponins are easily miscible in the water layer and are removed as water-soluble impurities such as sugar and inorganic salts; the total saponins The extract is directly applied to the macroporous resin column, which will also cause a part of the water-soluble saponin to be lost with water

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Technique for preparing malonyl ginsenoside, and application of medication in treating diabetes
  • Technique for preparing malonyl ginsenoside, and application of medication in treating diabetes
  • Technique for preparing malonyl ginsenoside, and application of medication in treating diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] Take 1kg of fresh ginseng (fibrous roots, branch roots, rhizomes, main roots, etc.) as raw material, chop it up, and use 15kg of 80% ethanol to extract it by cold soaking at room temperature for 3 times, each time for 8-16 hours. The extraction process should be fully stirred. The extract was recovered solvent under reduced pressure at 40°C, concentrated into an aqueous solution, extracted repeatedly with n-butanol: water, the aqueous layer solution was concentrated, then applied to a macroporous resin column, and eluted with water, 30%, 60% ethanol to obtain 60 The % ethanol part is total malonyl saponins, and the aqueous solution obtained by recovering ethanol is lyophilized and stored for later use, and then the preparation is developed. The shorter the experimental process of each step, the better, and the obtained samples should be stored at low temperature.

Embodiment 2

[0118] Take American ginseng (fibrous roots, branch roots, rhizomes, main roots, etc.) The solvent was recovered under reduced pressure, concentrated into an aqueous solution, extracted repeatedly with n-butanol: water, and the aqueous layer solution was lyophilized and stored at low temperature for future use. Take out the lyophilized sample and put it on the macroporous resin column, elute with water, 30% and 60% ethanol, and obtain 60% ethanol part as total malonyl saponins, and freeze-dry the aqueous solution obtained by recovering ethanol, and then develop the preparation. The shorter the experimental process of each step, the better, and the obtained samples should be stored at low temperature.

Embodiment 3

[0120] Get 60% ethanol phase (malonyl total saponins) 10g prepared in embodiment 1 or 2, dry loading, separate under reduced pressure with silica gel dry column (silica gel G 2Kg Qingdao ocean chemical factory production), use eluent (CHCl 3 -MeOH-H 2 O 65:35:10 lower layer) was washed to the bottom of the column, then cut off, checked with a thin layer of silica gel, and combined the components containing M-NR4.

[0121] Put the M-NR4 component on a silica gel dry column (600g, 300-400 mesh, 4.5×60cm), elute with chloroform-methanol-water (6:4:1), and check it with a thin layer of silica gel after breaking and cutting. H on M-NR4 + Type cation exchange resin column, continue to elute with ethanol, the eluate is concentrated under reduced pressure at 40°C, and the concentrated solution is recrystallized repeatedly with ethanol to obtain the compound malonyl notoginsenoside-R4.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
weightaaaaaaaaaa
Login to view more

Abstract

A process for preparing the malonyl ginsenoside used to prepare the medicine for treating diabetes includes pulverizing fresh ginseng, immersing in alcohol for extracting, vacuum recovering of solvent, concentrating to become aqueous solution, repeated extraction by n-butanol and water, concentrating, adsorbing by macroreticular resin column, eluting with alcohol, and recovering alcohol. A monoacyl arasaponin-R4 malonate and its medical application are also disclosed.

Description

technical field [0001] The present invention relates to medicines for treating diabetes, and in particular discloses a preparation process of malonyl ginsenoside and its medicinal use for treating diabetes, and also provides a new malonyl ginsenoside-R4 compound and its medical application The use belongs to the technical field of traditional Chinese medicine. Background technique [0002] Malonyl ginsenosides (Malonyl-ginsenosides) involved in the present invention are also called malonyl ginsenosides, mainly including M-Rb 1 , M-Rb 2 , M-Rc, M-Rd and M-NR4 series compounds. [0003] At present, in the commercial production of malonyl ginsenosides, when the total saponins are extracted with n-butanol, these saponins are easily miscible in the water layer and are removed as water-soluble impurities such as sugar and inorganic salts; the total saponins When the extract is directly applied to the macroporous resin column, part of the water-soluble saponins will also be lost...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07J9/00
Inventor 孙光芝李向高王继彦刘志孙晓波徐慧波
Owner JILIN AGRICULTURAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap