Extract product rich in gen-seng saponin in monosaccharide, preparation method and application
A technology of extract and ginseng, which is applied in the field of extract rich in ginseng monosaccharide saponins, can solve the problems of low price of Rh2 monomer, difficulty in wide application in the pharmaceutical industry, etc., and achieve the effect of simple preparation and favorable clinical application
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preparation example 1
[0016] Preparation Example 1: Preparation of an extract rich in ginseng monosaccharide saponins
[0017] Weigh 50kg of glycerin, add it to the reaction kettle, start stirring, heat the oil bath to 65°C, weigh 5kg of total saponins of American ginseng stems and leaves, and 5.5kg of sodium hydroxide, and add them to the reaction kettle in turn, and the heating rate is controlled at about 10-15°C / 10min, when the temperature rises to 195-205°C, stop heating and keep it for 20 minutes, then add the reactant to 15 times the amount of water, stir while adding, the saponin hydrolyzate forms grayish white precipitate, stand still, centrifuge, use Wash with ionized water until the filtrate does not show alkalinity, the pH value of the extract is measured at 6.5, take it out, and dry to obtain 3.35 kg of alkali degradation product.
[0018] Take 0.50kg of the alkali degradation product, heat and dissolve it with absolute ethanol, add silica gel powder, mix and mix the sample, dry the mi...
preparation example 2
[0020] Preparation Example 2: Preparation of an extract rich in ginseng monosaccharide saponins
[0021] Weigh 0.50 kg of the alkali degradation product in Preparation Example 1, heat and stir with absolute ethanol to dissolve, add alumina powder, mix and mix the sample, dry the mixed sample, pulverize, and sieve for chromatographic separation.
[0022] Weigh 15 kg of chromatographic alumina, add it to the chromatographic column, put the mixed sample on the upper part of the chromatographic column, elute with chloroform: methanol: ethyl acetate (9: 1: 20) eluent, collect the rich Containing ginsenosides Rh1, Rh2, and Rk2, the solvent was recovered to obtain 130 g of extract. As determined by high performance liquid chromatography, it contains 68.67% ginsenosides, including 53.45% Rh2, 11.82% ginsenoside Rh1 and 3.40% ginsenoside Rk2.
preparation example 3
[0023] Preparation Example 3: Preparation of an extract rich in ginseng monosaccharide saponins
[0024] Weigh 0.50 kg of the alkali degradation product in Preparation Example 1, heat and stir with absolute ethanol to dissolve, add alumina powder, mix and mix the sample, dry the mixed sample, pulverize, and sieve for chromatographic separation.
[0025] Weigh 15 kg of diatomaceous earth for chromatography, add it to the chromatography column, put the mixed sample on the upper part of the chromatography column, and elute with chloroform: methanol: ethyl acetate (9: 1: 10) eluent, The fraction rich in ginsenosides Rh1, Rh2 and Rk2 was collected, and the solvent was recovered to obtain 42 g of ginsenosides extract. As determined by high performance liquid chromatography, it contains 94.81% ginsenosides, including 93.15% Rh2, 0.54% ginsenoside Rh1, and 1.12% ginsenoside Rk2.
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