Plasmin producing strain and method for producing plasmin

A fibrinolysis and strain technology, applied in microorganism-based methods, biochemical equipment and methods, enzymes, etc., can solve the problems of high price, shortage of sources, large side effects, etc., and achieve the effect of low cost and strong feasibility

Inactive Publication Date: 2006-06-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research and development of thrombolytic agents are quite active, but the thrombolytic drugs used in clinical pr...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Bacillus sp.nov. SK006 was fermented under the following conditions,

[0021] Seed culture: seed culture medium (by mass volume percentage): peptone 1%, glucose 1%, yeast extract 0.5%, Na 2 HPO 4 12H 2 O 1.2%, MgSO 4 ·7H 2 O 0.03%, adjusted to pH 7.2, sterilized at 121°C for 20min, 180rpm, cultured at 37°C for 16-18h.

[0022] Liquid fermentation culture: liquid fermentation medium (by mass volume percentage): peptone 1.5%, glucose 1.5%, Na 2 HPO 4 12H 2 O 1.5%, NaH 2 PO 4 2H 2 O 0.13%, MgSO 4 ·7H 2 0.1%, antifoaming agent polyoxyethylene oxypropylene triglyceride 0.03%, 121 ℃ of sterilization 20min, adjust pH7.2; l) In the liquid fermentation medium, the culture conditions are: stirring speed 180rpm, control ventilation: 9L / min for 0~12h, 10.5L / min for 12~24h, 12L / min for 24~36h; culture temperature 37℃; The culture time was 36 hours, and the enzyme activity of the fermented liquid was 610.6u / ml. (based on urokinase).

Embodiment 2

[0024] Bacillus sp.nov. SK006 was fermented under the following conditions,

[0025] Seed culture: seed culture medium (by mass volume percentage): peptone 1%, glucose 1%, yeast extract 0.5%, Na 2 HPO 4 12H 2 O 1.2%, MgSO 4 ·7H 2 O 0.03%, adjusted to pH 7.2, sterilized at 121°C for 20min, 180rpm, cultured at 37°C for 16-18h.

[0026]Liquid fermentation culture: liquid fermentation medium (by mass volume percentage): peptone 2.5%, glucose 2.5%, Na 2 HPO 4 12H 2 O 2.5%, NaH 2 PO 4 2H 2 O 0.13%, MgSO 4 ·7H 2 0.1%, antifoaming agent polyoxyethylene oxypropylene triglyceride 0.03%, 121 ℃ of sterilizations 20min, adjust pH7.2, seed culture medium is inserted into 25 liters of fermenters by volume percentage 5% (filling volume 15 l) In the liquid fermentation medium, the culture conditions are: stirring speed 180rpm, control ventilation: 9L / min for 0~12h, 10.5L / min for 12~24h, 12L / min for 24~40h; culture temperature 40℃; The culture time was 40 hours, and the enzyme acti...

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Abstract

The present invention relates to plasmin producing strain and method of producing plasmin, and belongs to the field of food biotechnology. The present invention screens out one plasmin strain with thrombolysis capacity from fermented food shrimp paste, and the new strain is identified as one new variety of bacillus, and named as Bacillus sp.nov.SK006 and has the preservation number of CCTCC No. M205071. The present invention also provides method of producing plasmin with the said strain, and the method has less breeding work, low cost, high safety and reliability and may be used in large scale liquid fermentation production. The fermented product is non-toxic, and may be used in preparing capsule, granule, oral liquid or oral lozenge or as additive for health food or common food.

Description

technical field [0001] A strain for producing fibrinolytic enzymes and a method for producing fibrinolytic enzymes. The present invention relates to a strain for producing fibrinolytic enzymes——Bacillus sp.nov. SK006, whose preservation number is CCTCC NO: M 205071, It belongs to the field of food biotechnology. Background technique [0002] Cardiovascular and cerebrovascular diseases have always been known as the number one killer threatening human health. According to relevant survey reports, one Chinese dies of cardiovascular and cerebrovascular diseases every 24 seconds, and one Chinese becomes disabled every 13 seconds. There are more than 3 million people who die from cardiovascular and cerebrovascular diseases in my country every year, accounting for 50% of the total number of deaths in my country every year. Among the people who survived the disease, 75% lost their ability to work to varying degrees, and 4% were seriously disabled. Thromboembolic disease is the numb...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/68C12R1/07
Inventor 江波峰芳德华颖王璋
Owner JIANGNAN UNIV
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