Glume-cutting, anther-cutting, male-killing paddy rice cross breeding method

Abstract

The invention discloses a method of shearing glume and flower pesticide to kill masculinity for rice hybrid, with detail steps below: carrying out sexual hybridization with rice of same species, shearing 1 / 4-2 / 5 of the glume of not blooming in the hybrid female parent from the afternoon of last day to today, which shears off the flower pesticide but does not damage the chapiter, spraying clean water immediately to reach effect of killing masculinity; vibrating the rice treated with above steps before pollination to remove the flower pesticide from the chaff, then covering bags, pollinating the pollen of male parent into the glume, and getting the sexual hybridized seed of rice. The invention employs method of shearing plume and flower pesticide to kill masculinity for mass sexual hybridization of rice, which increases the hybridization efficiency, the plume and flower pesticide shearing rate and masculinity killing rate is greater than 98%, and is also characterized by the convenient, fast, and good effect, when comparing with normal sexual hybridization it can get more hybrid seeds and meet the demand of mass hybridization combination, which increases the hybridization efficiency greatly, homologous to the efficiency and effect of employing rice of male sterility line for hybridization.

Description

technical field [0001] The invention relates to the category of rice sexual hybridization methods, in particular to a rice hybridization method for cutting glumes, pruning herbs, and killing males. Background technique [0002] my country is a big grain-producing country, and rice is the largest crop in grain. In rice improvement and breeding, the most basic method of breeding new rice varieties is hybrid breeding. In this process, rice varieties are sexually hybridized, and the female parent kills males Most of them use the traditional "warm soup" to kill males and cut glumes manually, which is inconvenient, time-consuming and labor-intensive, and the efficiency of sexual hybridization is low. Especially in the research of biotechnology breeding, a large number of groups are required. Hybridization methods are difficult to achieve. For example, "Global Rice Molecular Breeding" constructs a large number of near-isogenic introduction lines, using 118 donor parents and 2 donor p...

AI Technical Summary

Problems solved by technology

[0002] my country is a big grain-producing country, and rice is the largest crop in grain. In rice improvement and breeding, the most basic method of rice new variety selection is hybrid breeding. In this process, rice varieties are sexually hybridized, and the female parent kills males Most of them use the traditional "warm soup" to kill males and cut glumes manually, which is inconvenient, time-consuming and labor-intensive, and the efficiency of sexual hybridization is low. Especially in the research of biotechnology breeding, a large number of groups are required. Hybridization methods are difficult to achieve. For example, "Global Rice Molecular Breeding" constructs a large number of near-isogenic introduction lines, using 118 donor parents and 2 donor parents. Each donor and donor parents are crossed and backcrossed. The backcross generation needs to cross 5,000 in one season, which cannot be achieved by conventional sexual crossing methods

The reason is that the artificial detasseling process is time-consuming and labor-intensive, and the efficiency of sexual hybridization is low