Glume-cutting, anther-cutting, male-killing paddy rice cross breeding method

A rice hybridization and rice technology, applied in the direction of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of low efficiency, inconvenient, time-consuming and labor-intensive sexual hybridization, so as to improve the efficiency of sexual hybridization and achieve good results , the effect of improving efficiency

Inactive Publication Date: 2006-07-12
江西省农业科学院水稻研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] my country is a big grain-producing country, and rice is the largest crop in grain. In rice improvement and breeding, the most basic method of rice new variety selection is hybrid breeding. In this process, rice varieties are sexually hybridized, and the female parent kills males Most of them use the traditional "warm soup" to kill males and cut glumes manually, which is inconvenient, time-consuming and labor-intensive, and the efficiency of sexual hybridization is low. Especially in the research of biotechnology breeding, a large numb...

Method used

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Examples

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Effect test

Embodiment 1

[0009] In this example, different rice varieties are used for sexual hybridization, and 1 / 4-3 / 5 of the rice varieties "Gumei No. 2", "M3122", and "IR72" used as female parents are cut off in the afternoon before the hybridization. For glumes, cut or cut off the anthers, and immediately spray clear water to prevent the pollen from dispersing. Before flowering the next day, use the thumb and index finger of your right hand to flick the tassels together a few times, and leave the glumes remaining in the glumes The anthers popped out, then bagged, and the pollen of the male parent "752" was used to confer on the female parent that had been pruned and pruned to kill the male, and the hybrid seeds could be obtained after 20-25 days, and the hybrid seeds were sown on the hybrid Next to the parents (female parent and male parent), identify the true and false hybrids. As a result, the true hybrids obtained by the rice hybrid method of clipping glumes and killing males are 98.4% on avera...

Embodiment 2

[0011] The rice varieties "I R58025", "Zihui 100", and "Teqing" used as hybrid female parents cut off 1 / 2 of the glumes on the day of hybridization and before flowering to cut off the anthers or cut off the anthers extending to the top, and Immediately spray clear water evenly to prevent pollen from dispersing, then bagging, after 20 days, check the selfing seed setting rate of the female parent after glume pruning and killing the male, the result is an average of 0.2%.

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Abstract

The invention discloses a method of shearing glume and flower pesticide to kill masculinity for rice hybrid, with detail steps below: carrying out sexual hybridization with rice of same species, shearing 1/4-2/5 of the glume of not blooming in the hybrid female parent from the afternoon of last day to today, which shears off the flower pesticide but does not damage the chapiter, spraying clean water immediately to reach effect of killing masculinity; vibrating the rice treated with above steps before pollination to remove the flower pesticide from the chaff, then covering bags, pollinating the pollen of male parent into the glume, and getting the sexual hybridized seed of rice. The invention employs method of shearing plume and flower pesticide to kill masculinity for mass sexual hybridization of rice, which increases the hybridization efficiency, the plume and flower pesticide shearing rate and masculinity killing rate is greater than 98%, and is also characterized by the convenient, fast, and good effect, when comparing with normal sexual hybridization it can get more hybrid seeds and meet the demand of mass hybridization combination, which increases the hybridization efficiency greatly, homologous to the efficiency and effect of employing rice of male sterility line for hybridization.

Description

technical field [0001] The invention relates to the category of rice sexual hybridization methods, in particular to a rice hybridization method for cutting glumes, pruning herbs, and killing males. Background technique [0002] my country is a big grain-producing country, and rice is the largest crop in grain. In rice improvement and breeding, the most basic method of breeding new rice varieties is hybrid breeding. In this process, rice varieties are sexually hybridized, and the female parent kills males Most of them use the traditional "warm soup" to kill males and cut glumes manually, which is inconvenient, time-consuming and labor-intensive, and the efficiency of sexual hybridization is low. Especially in the research of biotechnology breeding, a large number of groups are required. Hybridization methods are difficult to achieve. For example, "Global Rice Molecular Breeding" constructs a large number of near-isogenic introduction lines, using 118 donor parents and 2 donor p...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 陈大洲肖叶青邬文昌胡兰香吴小燕罗世友熊焕金邓伟刘兰英刘平洲盛国清
Owner 江西省农业科学院水稻研究所
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