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Method for apid determining niclosamide content in water sample

A rapid determination technology for niclosamide, applied in the preparation of test samples, sampling devices, color/spectral characteristic measurement, etc., can solve the problems of slow layering speed, high solubility, high extraction and enrichment ratio, etc., to achieve The requirements of the instrument and the quality of the operator are reduced, the molar absorptivity coefficient is increased, and the effect of increasing the cut-off area of ​​the molecule

Inactive Publication Date: 2006-08-09
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Before the present invention, relevant literature has reported to use esters to directly extract niclosamide, but if use low-carbon esters, the volatility is too strong, and the solubility in water is too large, it is not suitable for high extraction enrichment ratio; and high carbon number The density difference between the ester and water is too small, the layering speed is slow, and it is not suitable for rapid detection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Detection reagent preparation

[0025] Alkaline solution is made with sodium hydroxide and water, and its preparation method is: take 40 grams of sodium hydroxide solid and be made into 500 milliliters of aqueous solution.

[0026] The extract contains large cations and chloroform, and its preparation method is as follows: 0.03 g of cetyltrimethylammonium bromide (CTAB) is weighed and dissolved in 500 ml of chloroform.

[0027] (2) Visual colorimetric detection method:

[0028] Add 100 ml of water sample, 1 ml of alkaline solution, and 10 ml of extract into a 100 ml stoppered colorimetric tube; place it for 5 minutes after fully shaking, compare the color of the lower chloroform layer against the standard color scale, and obtain the water sample Approximate content of niclosamide in .

Embodiment 2

[0030] (1) Detection reagent preparation:

[0031] Alkaline solution is made with potassium hydroxide and water, and its preparation method is: take by weighing 56 grams of potassium hydroxide solid and be made into 500 milliliters of aqueous solution.

[0032] The extract contains large cations and chloroform, and its preparation method is as follows: 0.04 g of cetylpyridinium bromide (CPB) is weighed and dissolved in 500 ml of chloroform.

[0033] (2) Spectrophotometric detection:

[0034] Add 100 ml of water sample, 1 ml of alkaline solution, and 10 ml of extract to the separatory funnel. After fully oscillating, place it for 5 minutes (at this time, use a 1 cm cuvette filled with chloroform as a reference to adjust T=100%), add the lower layer of chloroform solution into a 1 cm cuvette, and use chloroform as a reference at a wavelength of 400 nanometers. Measure the absorbance of the blank and calculate the content of niclosamide in the water sample.

[0035] In the pre...

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Abstract

Present invention relates to a method for rapid measuring niclosamide content in water sample. Said invented liquid extract has large cation and organic solvent, adding aqueous alkali in water sample, niclosamide forming anion, adding liquid extract in water sample to make niclosamide anion and large cation forming ionic complex, organic solvent extracting ionic complex, determining niclosamide content by organic phase organic phase visual colorimetry visual colorimetry or visible spectrophotometric method. Said invented solves current detection method limitation of chromatography voltammetry method nonadaptability to express analysis and UV sensitivity insatiable to application rate analytic requirement spectrophotometry etc. Said invention can realize spectrophotometric method even visual colorimetry rapid determination.

Description

technical field [0001] The invention relates to a content determination method, in particular to a method for quickly determining the content of niclosamide in water samples. Background technique [0002] Schistosomiasis is a parasitic disease that seriously affects people's health and social and economic development. Schistosoma often parasitizes in snails and is very easy to infect humans through snails. Therefore, the most effective measure to eliminate schistosomiasis is to kill the snails, so as to prevent the transmission of schistosomiasis and ensure human health and safety. The most commonly used and effective drug for molluscicide is niclosamide, (scientific name is 5-chloro-salicyloyl-2'-chloro-4'nitroaniline, English name is Niclosamide), it is approved by the World Health Organization One of the main drugs used to kill molluscs. my country is currently using 50% niclosamide ethanolamine salt wettable powder. [0003] The dosage of 50% niclosamide ethanolamine...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N1/34G01N1/02
Inventor 嵇正平姜友富胡效亚高扬
Owner YANGZHOU UNIV
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