Method of raising insulin excretion by capejasmine extract through specificity inhibition UCP2

A gardenia extract and insulin secretion technology, applied in the fields of molecular endocrinology and cell biology, can solve the problems of lack of molecular biology research support, illusion, etc.

Inactive Publication Date: 2006-08-30
NANJING UNIV
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Problems solved by technology

Traditional Chinese medicine pays attention to "harmony of yin and yang", and believes that diseases are caused by imbalan

Method used

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  • Method of raising insulin excretion by capejasmine extract through specificity inhibition UCP2
  • Method of raising insulin excretion by capejasmine extract through specificity inhibition UCP2

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[0022] 1. Extraction and concentration of Gardenia 1.0kg of Gardenia was added to 10kg of distilled water, and decocted for 1.5 hours. After adsorption and extraction of D101 macroporous resin, distilled water was used to pass through the column to wash away impurities, and then eluted with 60% ethanol, and dried under reduced pressure at a low temperature of 70°C to form a powder. Finally, redissolve with distilled water to obtain 1L gardenia extract solution;

[0023] 2. Isolation and purification of mouse islets The mice used in the experiment are SPF grade wild-type mice and UCP2 gene knockout mice, strain c57 / B6, weighing 15-20g, and kept in this laboratory. Mice were sacrificed by cervical dislocation, laparotomy was performed, and the common bile duct into the duodenum was clamped with arterial clips. Liver upturned, free common bile duct. Perfuse about 2ml of pre-cooled Liberase RI enzyme working solution (0.167mg / ml) into the pancreas through the common bile duct to...

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Abstract

A method using the extrat of capejasmine fruit to specifically suppress the uncoupling protein 2 (UCP 2) for improving the secretion of insulin and treating diabetes B includes such steps as decocting capejasmine fruit, extracting by macroreticular resin, diluting with alcohol, vacuum freeze-drying to obtain extract, and using it to culture the pancreatic island cells of mouse for obviously stimulating the secretion of insulin.

Description

1. Technical field [0001] The invention belongs to the technical field of molecular endocrinology and cell biology, and specifically relates to the method of culturing mouse islet cells in vitro with gardenia extract, and stimulating insulin secretion by specifically inhibiting the expression of uncoupling protein 2 (UCP2). method. 2. Technical background [0002] Dysfunction of islet cells and insulin resistance often lead to type 2 diabetes. Islet cell dysfunction is a complex phenomenon, including the loss of glucose sensing, resulting in GSIS (glucose-stimulated insulin secretion) damage. [0003] UCP2 is a negative regulator of insulin secretion. First, it mediates the proton leakage of the mitochondrial inner membrane, which has been demonstrated by a series of studies covering proliposomes, mitochondria, and intact cells (Krauss S, et al. Proc. Natl. Acad. Sci, U.S.A. 99:118-122, 2002). In pancreatic β-cells, UCP2-mediated proton leakage reduces the amount of ATP ...

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Application Information

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IPC IPC(8): A61K36/744A61P3/10
Inventor 项阳张燕张红杰张辰宇刘畅孙国勋
Owner NANJING UNIV
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