Composite microorganism preparation of functional organic fertilizer and method for preparing same

A compound microbial inoculum and a technology for microbial inoculants are applied in the preparation of organic fertilizers, methods based on microorganisms, biochemical equipment and methods, etc., which can solve the problems such as the preparation method of organic fertilizers that have not been retrieved, and improve the quality of fruit and vegetable plants. , restore and maintain the effect of ecological balance

Inactive Publication Date: 2006-10-11
上海汇仁复合有机肥料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] After retrieving the relevant patent documents, the compound microbial bacterial agent of the organic fertilizer identical to the patent of the present invention and its preparation method have not yet been retrieved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] For ginger febrile disease of ginger, the preparation method comprises the following steps:

[0025] 1. Preservation of strains: Bacterial beads are grown on agar plates under suitable environmental conditions, and the bacterial cells are collected with suspension medium, and 0.2ml of bacterial suspension is added to each ampoule. Burn the cotton for the mouth plug, dry it in vacuum, seal it with fire, and store it in a -70°C refrigerator.

[0026] 2. Plate inoculation: Open the ampoule bottle of the preserved strain, add 0.1ml of nutrient fermentation liquid to prepare the bacterial suspension, inoculate the plate by streaking, culture in the incubator, and rejuvenate the bacterial beads with reduced vitality.

[0027] 3. Shake flask culture: put 150-200ml culture solution in a 500ml shake flask, sterilize, inoculate a single colony from a flat plate, or inoculate a spore suspension from a slope, and culture in a shaker.

[0028] 4. 20L seed tank culture: Inoculate th...

Embodiment 2

[0038] For banana head blight, the preparation method comprises the following steps:

[0039] 1. Preservation of strains: Bacterial beads are grown on agar plates under suitable environmental conditions, and the bacterial cells are collected with suspension medium, and 0.2ml of bacterial suspension is added to each ampoule. Burn the cotton for the mouth plug, dry it in vacuum, seal it with fire, and store it in a -70°C refrigerator.

[0040] 2. Plate inoculation: Open the ampoule bottle of the preserved strain, add 0.1ml of nutrient fermentation liquid to prepare the bacterial suspension, inoculate the plate by streaking, culture in the incubator, and rejuvenate the bacterial beads with reduced vitality.

[0041] 3. Shake flask culture: put 150-200ml culture solution in a 500ml shake flask, sterilize, inoculate a single colony from a flat plate, or inoculate a spore suspension from a slope, and culture in a shaker.

[0042] 4. 20L seed tank culture: Inoculate the seed tank fr...

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PUM

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Abstract

This invention discloses a recombinant microbial agent of a functional organic fertilizer and the preparation method thereof. The agent is mainly composed of bacillus subtilis, streptomyces jingyamsis, Lactobacillus bulgaricus, Bacillus cereus, bacillus megaterium and Enterococcus faecalis. The prepartation process comprises: bacterium preservation, plate inoculation, shake flask culture of seed solution, 20L seed fermentator culture, 500L fermentator production, centrifuging to collect the thallus, adding protective agent, emulsifying, lyophilizing, disintegrating, active bacteria counting, bacterial compability, vacuum-packing, low temperature storage at -20deg C, and sale for use.The invention is characterized in that, it can prevent and long-term control diseases and pests of fruits and vegetables, improve the quality of fruits and vegetables, and can efficiently resotre and keep the soil ecological balance.

Description

Technical field: [0001] The invention discloses a compound microbial bacterial agent of functional organic fertilizer and a preparation method thereof. Background technique: [0002] Countries such as Japan, the United States, and Australia have begun to use special microbial agents for high-temperature composting and fermentation of livestock and poultry manure, but their agents are all single bacteria or simple composite bacteria, which can only treat the bio-organic fertilizers. One or several indicators have been improved, and there is no compound microbial agent with obvious effect on the prevention and control of crop diseases and insect pests. [0003] There are some unreasonable problems in the development of microbial fertilizers at home and abroad, especially some domestic organic fertilizer production plants. Such as blindly thinking that the more types of bacteria added to the fertilizer, the better, and put the mutually antagonistic bacteria together; the ferme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/00C12R1/125C12R1/465C12R1/225C12R1/085C12R1/11
CPCY02W30/40
Inventor 陈汉民
Owner 上海汇仁复合有机肥料有限公司
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