Anticancer peptide extract of blood clam and its preparing method

Abstract

The present invention relates to anticancer blood clam peptide with high anticancer activity and its preparation process and belongs to the field of medicine technology. The anticancer blood clam peptide is prepared with blood clam as one kind of marine life and through serial technological process of extraction, precipitation, chromatographic separation, desalting, etc. The preparation process includes biological extract, staged ammonium sulfate precipitation, Superdex75 gel filtering and chromatographic separation, and Sephade G-25 column desalting. The screened out peptide molecules below 3000 Dal have optimal pharmaceutical effect and economic utility. The injection with the matter of the present invention has broad spectrum anticancer curative effect and low toxic side effect.

Description

technical field [0001] The present invention relates to a kind of clam anti-cancer peptide extract with high anti-cancer activity prepared from a series of technical measures such as extraction, precipitation, chromatography, desalination and the preparation method thereof, which belongs to field of medical technology. Background technique [0002] Extracts with anticancer activity can be prepared from marine organisms, especially clams, through technical treatment. After searching, Chinese patent application No. 98110478.9 discloses the technology of "an anti-cancer marine biological extract", which involves obtaining active substances such as glycoproteins from cockles or cockles by soaking in water and pulverizing them for low-temperature hydrolysis; Chinese patent application No. 03139763.8 discloses a "use and extraction method of polypeptide protein active substances in cockles". The extraction method disclosed in this patent application is to...

AI Technical Summary

Problems solved by technology

[0004] ①. The extracts in the prior art are glycoproteins or polypeptide proteins, that is, protein components rather than peptide compounds

[0005] ②. The extraction method in the prior art is a conventional preparation method. The conventional preparation method is outdated in technology, low in efficiency, and low in precision, and cannot carry out molecular weight fine differentiation

[0006] ③. The molecular weight of the prior art is greater than 3000Dal, and the pharmacological effect and economic and technical effect of the protein with large molecular weight are relatively poor, which is not conducive to the preparation of injections for clinical use

[0007] ④. The existing technology adopts centrifugal extraction under general conditions, and the centrifugal extraction process is difficult to ensure the activity of the extract, and the effect is poor

[0008] ⑤. The extraction steps of the existing technology are relatively extensive, and the extract contains various impurities, so it is not suitable for direct use in fields with high purity requirements