Anticancer peptide extract of blood clam and its preparing method

A technology of extract and anticancer peptide, which is applied in the field of clam anticancer peptide extract and its preparation, can solve the problems of low precision, backward technology and poor effect, and achieve the effect of accurate precision, advanced technology and high efficiency

Inactive Publication Date: 2006-11-08
青岛海生肿瘤医院
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Problems solved by technology

[0004] ①. The extracts in the prior art are glycoproteins or polypeptide proteins, that is, protein components rather than peptide compounds
[0005] ②. The extraction method in the prior art is a conventional preparation method. The conventional preparation method is outdated in technology, low in efficiency, and low in precision, and cannot carry out molecular weight fine differentiation
[0006] ③. The molecular weight of the prior art is greater than 3000Dal, and the pharmacological effect and economic and technical effect of the p

Method used

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Embodiment Construction

[0031] The present invention will be further described below in conjunction with the examples given.

[0032] Weigh 600g of fresh clam raw material caught in the sea, wash the sludge with tap water, move it to a class 10,000 clean room (temperature control below 10°C), remove the shell, take out the soft part, wash it with purified water until there is no blood stain, and freshly prepare the injection Wash with water (required to be cooled below 10°C) three times, drain the water, and mash the soft tissue with a masher (particle size 1mm). Add freshly prepared water for injection (cooled to below 10°C) to the homogenized net material in a ratio of 1:1 (w / w), and stir slowly for 4 hours under low temperature conditions (60 rpm to maintain the temperature of the extract at about 4°C) , a refrigerated centrifuge (2000 rpm) to remove slag and collect the separation liquid, which is then centrifuged in a tubular centrifuge (16000 rpm), the separation liquid is collected in a stainl...

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Abstract

The present invention relates to anticancer blood clam peptide with high anticancer activity and its preparation process and belongs to the field of medicine technology. The anticancer blood clam peptide is prepared with blood clam as one kind of marine life and through serial technological process of extraction, precipitation, chromatographic separation, desalting, etc. The preparation process includes biological extract, staged ammonium sulfate precipitation, Superdex75 gel filtering and chromatographic separation, and Sephade G-25 column desalting. The screened out peptide molecules below 3000 Dal have optimal pharmaceutical effect and economic utility. The injection with the matter of the present invention has broad spectrum anticancer curative effect and low toxic side effect.

Description

technical field [0001] The present invention relates to a kind of clam anti-cancer peptide extract with high anti-cancer activity prepared from a series of technical measures such as extraction, precipitation, chromatography, desalination and the preparation method thereof, which belongs to field of medical technology. Background technique [0002] Extracts with anticancer activity can be prepared from marine organisms, especially clams, through technical treatment. After searching, Chinese patent application No. 98110478.9 discloses the technology of "an anti-cancer marine biological extract", which involves obtaining active substances such as glycoproteins from cockles or cockles by soaking in water and pulverizing them for low-temperature hydrolysis; Chinese patent application No. 03139763.8 discloses a "use and extraction method of polypeptide protein active substances in cockles". The extraction method disclosed in this patent application is to...

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Application Information

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IPC IPC(8): A61K38/17A61K9/19A61K35/618A61P35/00
Inventor 陈守国王春波姚如永韩彦弢韩志武李光耀初晓杨秀
Owner 青岛海生肿瘤医院
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