Hydrophilic and phydrophobic mode sheet base special for biological chip

Abstract

This invention relates to a special hydrophilic and drain mode film base for biochips by providing a special hydrophilic and drain mode film base of a biochip having hydrophilic points in the same area and same size in a modified drain surface (thickness of the drain layer is 2-5mum) on a glass slide, silicon plate or other carriers, in which, shape, area, density and array arrangement can be decided according to the needs and the shape of the points are generally round, the film base has a substrate having a drain layer on the top surface and array hydrophilic points are distributed on the drain layer.

Description

technical field [0001] The invention relates to a biochip substrate, in particular to a modified hydrophobic surface to construct hydrophilic dots with uniform area and size (the shape, area, density and arrangement of the dots can be determined according to actual needs) The biochip is dedicated to the hydrophilic and hydrophobic mode substrate. Background technique [0002] To make a biochip, in addition to special instruments, it is also necessary to choose a suitable solid support--a substrate, that is, a carrier material. As a chip substrate, the following requirements must be met: 1. There are active groups on the surface, which can be coupled with biomolecules; 2. Inertness (does not affect the function of biomolecules) and stability (including mechanical, chemical, physical, etc.); 3. , Good biocompatibility. Generally speaking, all kinds of chip bases choose correspondingly treated silicon wafers, glass wafers, ceramic wafers or polypropylene membranes, nitrocellu...

AI Technical Summary

Problems solved by technology

For the contact mode, a hydrophilic film base is usually selected, which is beneficial to transfer the sample to the film base during the contact process, but the hydrophilic film base has the following unavoidable shortcomings in use: 1. It leads to sample spot diffusion As a result, the spot area is relatively large, and high-density array points cannot be obtained, and cross-contamination between sample points is easily caused; 2. The quality of the sample point (reproducibility of sample point volume and area) is largely dependent on the sample distributor.

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