Method for measuring content of cozymase I and derivative thereof

Abstract

The disclosed content detection method for coenzyme I and its derivative comprises: preparing sodium pyrophosphate buffer with 8.0-11.0 pH value contained 0.01-0.3v% hydrazine hydrate; adding substrate solution and the target solution; with a spectrophotometer, after the maximal absorption peak, adding 3alpha-steroid dehydrogenase till absorbance stopping; calculating the content according to absorbance calculation.

Description

technical field

[0001] The invention relates to a method for determining the content of coenzyme I and its derivatives. Background technique

[0002] A change in the characteristic absorption spectrum may occur when the oxidized coenzyme I and the reduced coenzyme I are bidirectionally converted. Under certain conditions, it can change linearly with the concentration of the analyte. Many chemical analysis methods are realized based on this principle, but there are many defects in the chemical properties of oxidized / reduced coenzyme I. The chemical properties of oxidized / reduced coenzyme I derivatives have changed greatly, (mainly manifested as changes in maximum absorption peaks, increases in molar absorbance, changes in reaction Km values, and greatly increased solution stability and stable pH range.) The maximum absorption wavelength of the reduced coenzyme I derivatives is between 340-405nm, and the oxidation-reduction reaction participated by the oxidized / reduced coenzy...