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Culture medium for inducing garlic somatic embryo

A medium and basic medium technology, applied in plant cells, horticulture, botany equipment and methods, etc., can solve the problem of low induction rate of somatic embryos

Inactive Publication Date: 2006-12-27
JIANGSU XUHUAI DISTRICT XUZHOU AGRI SCI INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

People use the rapid reproduction method of embryoid body generation, and coat the surface with a layer of special organic substances, etc., the so-called "artificial seeds", which provide a new way for rapid production of seedlings. The rate of induction of somatic embryos from wounded tissue is low

Method used

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  • Culture medium for inducing garlic somatic embryo

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Medium formula:

[0025] MS 1L

[0026] 6-BA 1.0mg

[0027] 2,4-D 0.1mg

[0028] CH200mg

[0029] Sucrose 20g

[0030] Agar 6g

Embodiment 2

[0032] Medium formula:

[0033] MS 1L

[0034] 6-BA 1.0mg

[0035] 2,4-D 1.0mg

[0036] CH200mg

[0037] Sucrose 20g

[0038] Agar 6g

Embodiment 3

[0040] Medium formula:

[0041] MS 1L

[0042] 6-BA 0.1mg

[0043] 2,4-D 1.0mg

[0044] CH200mg

[0045] Sucrose 20g

[0046] Agar 6g

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Abstract

The invention discloses a culture medium which can induce the somatic cell from garlic idiosome callus, which is prepared by dissolving 6-benzyl purine 6-BA, 2, 4-dichlorophenoxyacetic acid 2, 4-D, casein hydrolysate CH, sucrose and gelose in minimum medium MS. The advantage is that it has a high inductivity and so is suitable for plant production of garlic.

Description

technical field [0001] The invention belongs to garlic tissue culture technology, in particular to a culture medium for inducing somatic embryos from garlic embryogenic callus. Background technique [0002] The callus formed by inducing explant cell division in plant tissue culture is loose callus (non-embryogenic callus) and embryogenic callus. Only under suitable conditions can the embryogenic callus continue to redifferentiate, form embryoid bodies, and then form shoots, roots, and regenerate complete plants. Embryoid bodies, also known as somatic embryos or somatic embryos, are embryo-like structures produced by somatic cells. Their development process is similar to zygotic embryos. They are divided into proembryos, globular embryos, heart-shaped embryos, torpedo-shaped embryos, and cotyledon embryos. several stages. The embryoid body regeneration system is not only the most ideal gene transformation recipient system, but also one of the eff...

Claims

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Application Information

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IPC IPC(8): C12N5/04A01H4/00
Inventor 张允刚
Owner JIANGSU XUHUAI DISTRICT XUZHOU AGRI SCI INST