Biomolecule mobilization substrate, bio-chip and biological sensor

A biomolecule and biochip technology, applied in biochemical equipment and methods, microbial determination/inspection, biological testing, etc., can solve the problems of time-consuming and low efficiency, achieve long life, not easy to inactivate, and reduce time effect of change

Inactive Publication Date: 2007-01-10
ORMON CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in this lipid bilayer formation method, both the process of forming a peptide monomolecular membrane and the process of forming a lipid bilayer are very time-consuming procedures, and the efficiency is low.

Method used

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  • Biomolecule mobilization substrate, bio-chip and biological sensor
  • Biomolecule mobilization substrate, bio-chip and biological sensor
  • Biomolecule mobilization substrate, bio-chip and biological sensor

Examples

Experimental program
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Embodiment 1

[0073] figure 1 It is a diagram schematically showing the structure of the biochip 11 (that is, the biomolecule-immobilized substrate 12 on which receptors are immobilized) of the present invention. In the biomolecule immobilization substrate 12 , as described in detail below, the lipid bilayer 24 is tethered on the chip substrate 21 with the metal layer 22 formed on the surface through the hydrophilic monomolecular membrane 23 . In addition, the biochip 11 immobilizes the biorecognition molecule 27 on the lipid bilayer 24 of the biomolecule immobilization substrate 12 , and the receptor 28 is immobilized on the biorecognition molecule 27 .

[0074] The chip substrate 21 is a thin plate formed of a light-transmitting material such as glass or quartz. A plurality of metal particles are fixed on the chip substrate 21 to form a metal layer 22 .

[0075] The metal particles forming the metal layer 22 are nano-level inorganic metal particles such as Au and Ag with a diameter of s...

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Abstract

The invention provides new biomolecule immobilized substrate and biochip which use hydrophilic monomolecular film and lipid bilayer to immobilize biomolecule on a chip substrate. On the transparent chip substrate 21, metal layer 22 consisting of Au particulate is formed. Further on it, monomolecular film 23 consisting of X-(CH2)n-OH (X:thiol group) is formed utilizing self assembly. On the monomolecular film 23, lipid bilayer 24 consisting of phospholipid is formed utilizing self assembly. Both the monomolecular film 23 and the lipid bilayer 24 are rather flexibly combined by hydrogen bond. On this lipid bilayer 24, receptor 28 is immobilized through biosensing molecule 27.

Description

technical field [0001] The invention relates to a biomolecule immobilized substrate, a biochip and a biosensor. More specifically, the present invention relates to the immobilization of biomolecules on substrates using hydrophilic monolayers and lipid bilayers. Background technique [0002] (About biosensing) [0003] For biochips and quantum chips that arrange biomolecules two-dimensionally on chip substrates, their applications in the medical-environmental, electronic and other fields are currently being explored. Especially in the field of treatment-diagnosis and the study of biological mechanisms, the protein chip with a large number of protein molecules (protein) arranged two-dimensionally on the chip substrate is ideal for disease diagnosis, health diagnosis, individual identification, and biological system analysis. very necessary. [0004] For example, in order to understand biological systems, it is necessary to have a clear understanding of the interaction netwo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N33/543C12Q1/00C25D1/00
Inventor 松下智彦西川武男山下英之池田正哲青山茂和沢铁一濑崎浩史
Owner ORMON CORP
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