Stable polypeptide composition and application thereof

A polypeptide composition and stable technology, applied in the field of polypeptide raw material preparation, can solve problems such as easy generation of impurities, difficulties in subsequent formulations, and impact on use efficacy, and achieve good safety, not easy to inactivate, and stable structure

Active Publication Date: 2017-11-03
ZHUHAI UNITED LAB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The researchers of the present invention found that after nonapeptide-1, acetyl hexapeptide-8, and acetyl octapeptide-3 are mixed with other polypeptides, impurities are easily produced during the raw material placement process, which leads to difficulties in the formulation of subsequent preparations and affects their efficacy.

Method used

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  • Stable polypeptide composition and application thereof
  • Stable polypeptide composition and application thereof
  • Stable polypeptide composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Preparation of Acetyl Hexapeptide-8 and Palmitoyl Pentapeptide-4 Composition 1

[0046] (1) The formula is shown in Table 1:

[0047] Table 1

[0048] Acetyl Hexapeptide-8

[0049] (2) Preparation process: add 80g of Tween-80 and 500g of purified water into the batching tank, and stir well. Add 5g of palmitoyl pentapeptide-4 and 10g of acetyl hexapeptide-8 in turn. After continuous stirring and dissolving at 1000rpm, dissolve 2g of lipoic acid and 3g of methionine with 300g of purified water, add them to the batching tank, and then add 10g of caprylic acid. alcohol, 20g phenoxyethanol, stir well. Finally, slowly drop 2mol / L sodium hydroxide solution into the formula reaction tank, increase the stirring speed to 1500rpm, adjust the pH to 5.5, and then fix the weight to 1000g with purified water. Filtered through sterile PES filter, the solution is clear and transparent.

Embodiment 2

[0050] Example 2: Preparation of Acetyl Hexapeptide-8 and Palmitoyl Pentapeptide-4 Composition 2

[0051] (1) The formula is shown in Table 2:

[0052] Table 2

[0053]

[0054]

[0055](2) Preparation process: add 5g of Tween-80 and 500g of purified water into the batching tank, and stir well. Add 0.1 g of palmitoyl pentapeptide-4 and 0.5 g of acetyl hexapeptide-8 in turn. After continuous stirring and dissolving at 1000 rpm, dissolve 0.1 g of lipoic acid and 0.1 g of methionine with 300 g of purified water, and add them to the batching tank. Add 0.5 g of caprylyl glycol and 4.5 g of phenoxyethanol, and stir well. Finally, slowly drop 2mol / L sodium acetate solution into the formula reaction tank, increase the stirring speed to 1500rpm, adjust the pH to 5.5, and then use purified water to fix the weight to 1000g. Filtered through sterile PES filter, the solution is clear and transparent.

Embodiment 3

[0056] Example 3: Preparation of Acetyl Hexapeptide-8 and Palmitoyl Pentapeptide-4 Composition 3

[0057] (1) The formula is shown in Table 3:

[0058] table 3

[0059] Acetyl Hexapeptide-8

[0060] (2) Preparation process: add 8g of Tween-80 and 500g of purified water into the batching tank, and stir well. Add 0.1g of palmitoyl pentapeptide-4 and 0.5g of acetyl hexapeptide-8 in turn. After continuous stirring at 1000rpm, dissolve 10g of carnosine and 40g of cysteine ​​with 300g of purified water, add them to the batching tank, and then add 2g of glycerol. , 8g phenoxyethanol, stir well. Finally, slowly drop 2mol / L sodium carbonate solution into the formula reaction tank, increase the stirring speed to 1500rpm, adjust the pH to 6.0, and then fix the weight to 1000g with purified water. Filter through sterile PES filter, the solution should be clear and transparent.

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Abstract

The invention discloses a stable polypeptide composition and application thereof. The polypeptide composition consists of sulfhydryl-containing polypeptides, water-insoluble polypeptides, a solubilizer, a stabilizing agent, a preservative, water and a pH value regulating agent. The invention is a result unexpectedly discovered by an inventor that when the stabilizing agent and the preservative simultaneously exist, the problem of easiness in impurity generation in the raw material placing process of a mixed aqueous solution of the sulfhydryl-containing polypeptides and the water-insoluble polypeptides is better solved; the prepared polypeptide composition is good in raw material quality, photo-stable, capable of placement for a long term at a room temperature and easy for the preparation of a subsequent preparation.

Description

technical field [0001] The invention belongs to the technical field of polypeptide raw material preparation, and particularly relates to a stable polypeptide composition and its application. Background technique [0002] Polypeptide is a class of biologically active substances condensed by two or more amino acids and linked by peptide bonds. It is an intermediate product of protein, and its molecular structure is between amino acid and protein. In vivo, these active biological polypeptides not only exist in the form of polypeptide molecular structure and natural conformation, but also play important biological effects in the body in the form of natural biological activity, and play an important role in the growth, development and metabolism of organisms. vital biological role. Active biological peptides also play a role in the natural aging defense and nursing processes of skin tissue cells such as proliferation, cell chemotaxis and migration, repair and regeneration, angio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/64A61Q19/00A61K38/08A61K38/07A61P17/00A61K8/34A61K8/37A61K8/42A61K8/44A61K8/46A61K8/67A61K8/97A61K8/23A61K47/02A61K47/10A61K47/18A61K47/20A61K47/22A61K47/46A61K8/35
CPCA61K8/23A61K8/345A61K8/347A61K8/35A61K8/37A61K8/42A61K8/44A61K8/463A61K8/64A61K8/676A61K8/678A61K8/97A61K38/07A61K38/08A61K47/02A61K47/10A61K47/18A61K47/183A61K47/20A61K47/22A61K47/46A61K2800/10A61K2800/5922A61Q19/00A61K2300/00
Inventor 杨祺周翠曹春来黄有泉许文威王康钊安鹏飞黄振彪
Owner ZHUHAI UNITED LAB
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