Unlock instant, AI-driven research and patent intelligence for your innovation.

Fi-C31 integrase modified TAT and NLS polypeptide and its use

An integrase and protein technology, applied in the field of transgenic, can solve the problems of cytotoxicity, mutation, low gene transfer efficiency, etc., and achieve the effect of improving transfer efficiency and safety.

Inactive Publication Date: 2007-01-17
FUDAN UNIV
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Non-viral vectors such as naked DNA plasmid vectors are considered to be relatively safe and easy to prepare and apply for gene transfer, but there is also the potential for random integration of vector DNA into cell chromosomes to cause gene mutations, as well as long-term expression of vector genes. Toxic to cells; in addition, the gene transfer of plasmid DNA is affected by different cell types, and in some cells, only a very low gene transfer efficiency

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fi-C31 integrase modified TAT and NLS polypeptide and its use
  • Fi-C31 integrase modified TAT and NLS polypeptide and its use
  • Fi-C31 integrase modified TAT and NLS polypeptide and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0030] Below in conjunction with specific example, further set forth the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental method that does not indicate specific conditions in the following examples is usually according to conventional conditions such as people such as Sambrook, molecular cloning: the conditions described in the laboratory manual (New York: Cold Spring Harbor Laboratory Press, 1989), and such as David etc. Human, conditions described in Cellular Experiment Manual (New York: Cold Spring Harbor Laboratory Press, 1998).

[0031] 1. Construction, expression and purification of three ΦC31 fusion proteins:

[0032] The expression and purification of the three fusion proteins of ΦC31 use basically the same affinity chromatography method, and their different modifications are shown in figure 1 Among the symbols indicated b...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

PHIC31 integrase protein with TAT and NLS polypeptide modification and its use are disclosed. It consists of PHIC31 integrase protein TAT-PHIC31 and PHIC31 integrase protein TAT-PHIC31-NLS. It's safe and efficient.

Description

technical field [0001] The invention belongs to the field of transgenic technology, and specifically relates to a new modified ΦC31 integrase and its application in gene transfer. Background technique [0002] Using gene transfer technology to effectively integrate the target gene site-specifically into the genome of mammalian cells, thereby avoiding random insertion of foreign genes into the genome, is an important unsolved problem in gene therapy and transgenic animal research. In recent years, the clinical treatment of severe combined immunodeficiency (SCID-XI) has achieved success by using retroviral vectors. Subsequent treatment patients developed leukemia cancer, mainly due to the random insertion of foreign genes into cell chromosomes by the gene transfer technology of viral vectors, causing gene mutations (S.Hacein-Bey-Abina, et al.A serious adverse event after successful gene therapy for X-linked severe combined immunodeficiency. N. Engl. J. Med. 2003, 346, 1185-11...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N9/00C12N15/52C12N15/09C12N1/21
Inventor 张茂祥朱焕章陈金中薛京伦
Owner FUDAN UNIV