Datura flower total steroid, and its preparing method and use

A kind of ginseng and steroid technology, applied in the preparation of steroids, chemical instruments and methods, pharmaceutical formulations, etc., can solve the problem of no anti-tumor, and achieve the effect of strong anti-tumor effect

Inactive Publication Date: 2007-03-28
卡南吉医药科技(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no literature reports on anti-tumor aspects of single herbs and effective parts

Method used

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  • Datura flower total steroid, and its preparing method and use
  • Datura flower total steroid, and its preparing method and use
  • Datura flower total steroid, and its preparing method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Get 100 grams of Dajinhua, add 1 liter of 95% ethanol at a time to reflux and extract for 1 hour, extract 4 times, combine the extracts, and evaporate the solvent to obtain the Dajinhua extract, which is dissolved in 50% ethanol and used Extract this solution with petroleum ether for 4 times, dilute the non-petroleum ether layer with water to 1 time, pass through D101 macroporous adsorption resin after mixing, elute water-soluble substances with water, elute non-steroidal substances with 25% ethanol, and then wash with 95 % ethanol eluate, collect 95% ethanol eluate, evaporate ethanol to get concentrated solution, extract concentrated solution 3 times with ethyl acetate, combine ethyl acetate layer, wash ethyl acetate layer with PH13 sodium hydroxide aqueous solution 3 times, wash the organic phase 3 times with water, wash the ethyl acetate layer 3 times with PH1 aqueous hydrochloric acid solution, wash the ethyl acetate layer with water to neutrality, evaporate the ethy...

Embodiment 2

[0053] 25 grams of Dajinhua, add 200 milliliters of 50% ethanol for reflux extraction for 0.5 hours each time, extract 3 times, combine the extracts, evaporate the ethanol to obtain an aqueous solution, then extract the aqueous solution 3 times with ethyl acetate, combine the ethyl acetate layers, Wash the ethyl acetate layer with pH 13 alkaline water for 4 times, wash the organic phase with water for 3 times, wash the ethyl acetate layer with pH 1 aqueous hydrochloric acid solution for 3 times, wash the ethyl acetate layer with water to neutrality, and wash the ethyl acetate layer Concentrate to 10 ml, mix with alumina, filter after standing, wash activated carbon with 95% ethanol until colorless, collect ethanol eluate and evaporate to dryness to obtain 0.09 g of total steroid extract of Daphnia japonica, containing 40.68% steroids.

Embodiment 3

[0055] Take 20 grams of daphnia japonica, add 200 ml of 85% ethanol each time for reflux extraction for 2 hours, extract 3 times, combine the extracts, and evaporate the solvent to obtain a crude extract. The crude extract was dissolved with 200 milliliters of alkaline water of pH 11, and the aqueous alkaline solution was extracted 5 times with ethyl acetate, the ethyl acetate layers were combined, the ethyl acetate layer was washed 3 times with alkaline water of pH 11, and the organic phase was washed 3 times with water, The ethyl acetate layer was washed 3 times with aqueous hydrochloric acid solution of pH 3, and the ethyl acetate layer was washed with water to neutrality, and the ethyl acetate layer was evaporated to dryness to obtain 0.5 g of total steroid extract of Dajina flower, containing 48.86% steroids.

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Abstract

The present invention discloses a datura flower total steroid extract and its preparation method. Said invention uses datura flower as raw material, uses organic solvent as extraction solvent and adopts conventional process to make extraction so as to obtain datura flower total steroid extract. Said extract can obviously inhibit growth of tumor, can be used for preparing anti-tumor medicine.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine. In particular, it relates to a total steroid extract of daphnia japonica, a preparation method of the extract and an application in preparation of drugs for treating tumors. Background technique [0002] Tumor is a disease that seriously threatens human life and health. According to the World Health Organization survey, the proportion of cancer patients in the world accounts for about 6.89‰, while the number of new cancer patients is 1.78‰ every year, and the number of survivors is nearly 40 million. According to statistics from the Ministry of Health, the prevalence of malignant tumors in China has reached 1.15-1.27‰, and the death due to malignant tumors ranks second among all causes of death. Although great progress has been made in the treatment of malignant tumors, there is still a lack of ideal therapeutic drugs to meet clinical needs. Malignant tumors are still regard...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/81A61P35/00C07J75/00C07J9/00C07J17/00C07J71/00
Inventor 王晓川吴威巍王作鹏
Owner 卡南吉医药科技(上海)有限公司
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