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Biosensors for detecting bond rupture

A biosensor and bond breaking technology, applied in the field of biosensors, can solve problems such as detecting bond breaks

Inactive Publication Date: 2007-04-11
IND RES LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Detects bond breakage and thus confirms the presence of the target substance

Method used

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  • Biosensors for detecting bond rupture
  • Biosensors for detecting bond rupture
  • Biosensors for detecting bond rupture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0061] Figure 5 shows an example of one embodiment of a biosensor using an SPR device to detect bond breaking. The biosensor includes an oscillation source 50 , a delay line 51 , a fluid channel 52 , a surface 53 , a reflection layer 54 , an incident light beam 55 and a reflected light beam 56 . A light detector (not shown) is also included which detects angular changes in the reflected light beam 56 . In this embodiment, oscillator 50 is a 10 MHz converter connectable to a waveform generator.

[0062] This example shows both surface immobilization and bond breaking scanning. These are monitored in situ by combining both SPR detection and acoustic waveform sensing into thin layer flow cells.

[0063]Surface 53, which in this example is a gold surface, provides a surface on which a self-assembled monolayer can be formed. In this example, 5 mg of biotin-PEO3-amine was dissolved in 250 ml of 0.1 M phosphate buffered saline (PBS) solution (pH 7.4). The amine and PBS solution w...

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PUM

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Abstract

A biosensor comprises a surface onto which bio-macromolecules are bound, the surface and bio-macromolecules being immersed in liquid, a bond rupture detector associated with the surface and arranged to detect the rupture of bonds between the bio-macromolecules and a target substance ( 6 ), and an oscillator ( 8 ) associated with the liquid and spaced from the surface and arranged to produce oscillations in the liquid to cause bonds between the bio-macromolecules and the target substance ( 6 ) to rupture.

Description

technical field [0001] The present invention relates to biosensors for the detection of various chemical and biological agents, and more particularly to biosensors for the detection of various chemical and biological agents using separate detectors and oscillators. Background technique [0002] In current New Zealand medical practice, when a patient is ill, swabs are taken and analyzed in a laboratory. For example, to determine a patient's influenza strain, an aliquot of the appropriate fluid is incubated with purified antibodies to characteristic molecules or markers on the surface of the influenza virus. The virus concentration can be determined with the aid of amplification reagents and secondary antibodies labeled with enzymes or radioisotopes. This analysis, although very sensitive, takes at least a few hours, but often takes much longer. Thus, it may take hours or even a day before an accurate diagnosis of the patient is available. There could be even longer delays ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/543G01N33/48G01N29/02C12Q3/00C12Q1/00C12M1/34G01N21/55G01N29/14G01N29/22G01N29/24
CPCG01N2291/0251G01N29/24G01N33/54373G01N2291/0423G01N29/14B82Y15/00G01N21/553G01N2291/0426G01N29/222B82Y30/00
Inventor Y·袁M·安德鲁斯W·M·阿诺德B·马洛
Owner IND RES LTD
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