Gastrodia elata plant extract for preventing senile dementia and its preparing method
A plant extract and senile technology, applied in the field of disease medicine, can solve problems such as intelligence enhancement, unreported Gastrodia elata plant extract, undiscovered, etc.
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Embodiment 1
[0047] 1. Extraction: 1 kg of Gastrodia elata, crushed and soaked in 50% ethanol for 3 times, 24 hours each time. Combine the extracts, recover ethanol and concentrate to a thick extract, add distilled water to heat and dissolve, cool to room temperature, let stand for 24 hours, centrifuge and filter to obtain a clarified filtrate.
[0048] 2. Refining: the above-mentioned filtrate is eluted by gradient elution with water and 10%, 20%, 30%, 40%, 50%, 75% ethanol through the treated HPD-100 macroporous adsorption resin, detected by HPLC, and collected rich in Fractions of palicine compounds were combined, concentrated under reduced pressure, and spray-dried to obtain 4.48 g of the plant extract. As determined by high performance liquid chromatography, the content of parisin compounds is 51.8%.
Embodiment 2
[0050] 1. Extraction: 1 kg of Gastrodia elata medicinal material, pulverized, added with 50% ethanol, soaked at 50°C for 3 times, 6 hours each time. Combine the extracts, recover ethanol and concentrate to a thick extract, add distilled water to heat and dissolve, cool to room temperature, let stand for 24 hours, centrifuge and filter to obtain a clarified filtrate.
[0051] 2. Refining: the above-mentioned filtrate is eluted by gradient elution with water and 10%, 20%, 30%, 40%, 50%, and 75% ethanol through the treated HP-20 type macroporous adsorption resin, detected by HPLC, and collected rich in Fractions of palicine compounds were combined, concentrated under reduced pressure, and spray-dried to obtain 4.42 g of the plant extract. As determined by high-performance liquid chromatography, the content of parisin compounds is 52.6%.
Embodiment 3
[0053] 1. Extraction: 1 kg of Gastrodia elata, a traditional Chinese medicine, was pulverized and soaked in 50% ethanol overnight, then heated and refluxed for 3 times, 3.0 hours each time. Combine the extracts, recover ethanol and concentrate to a thick extract, add distilled water to heat and dissolve, cool to room temperature, let stand for 24 hours, centrifuge and filter to obtain a clarified filtrate.
[0054] 2. Refining: the above-mentioned filtrate is washed with water, 10%, 20%, 30%, 40%, 50% and 75% concentration of methanol successively through the treated HP-20 macroporous adsorption resin, detected by HPLC, and collected rich Fractions containing parisin compounds were combined, concentrated under reduced pressure, and spray-dried to obtain 4.68 g of the plant extract. As determined by high performance liquid chromatography, the content of parisin compounds is 54.4%.
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