Method for capillary electrophoresis electrochemiluminescence detection of metoprolol and atenolol

A technology of capillary electrophoresis and luminescence detection, which is applied in the direction of chemiluminescence/bioluminescence, electrochemical variables of materials, and analysis by making materials undergo chemical reactions. It can solve the problems of high instrument cost and achieve low cost, high sensitivity and simple operation. Effect

Inactive Publication Date: 2007-07-11
CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the equipment required for chromatography-mass spectrometry is finely structured, and the cost of the equipment is relatively high

Method used

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  • Method for capillary electrophoresis electrochemiluminescence detection of metoprolol and atenolol
  • Method for capillary electrophoresis electrochemiluminescence detection of metoprolol and atenolol
  • Method for capillary electrophoresis electrochemiluminescence detection of metoprolol and atenolol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The detection of embodiment 1 metoprolol and atenolol standard substance

[0042] For the detection of metoprolol and atenolol standard products, the instrument used is as described above, and the detection steps are as follows:

[0043] 1. Preparation of standard solutions of metoprolol and atenolol

[0044] The stock solution concentration of metoprolol and atenolol was 1.0mmol / L. The stock solution was stored in a refrigerator at 4°C, protected from light.

[0045] 2.10.0mmol / L, NaH at pH8.5 2 PO 4 -Na 2 HPO 4 Preparation of buffer solution

[0046] First prepare NaH with a concentration of 10.0mmol / L 2 PO 4 solution and Na at a concentration of 10.0mmol / L 2 HPO 4 solution, the two were then mixed and adjusted to pH 8.5.

[0047] 3.100.0mmol / L, NaH at pH8.5 2 PO 4 -Na 2 HPO 4 Preparation of buffer solution

[0048] First prepare NaH with a concentration of 100.0mmol / L 2 PO 4 solution and a concentration of 100.0mmol / L Na 2 HPO 4 solution, and then...

Embodiment 2

[0054] Example 2 Simultaneous Separation and Detection of Metoprolol and Atenolol Standards

[0055] For the simultaneous separation and detection of metoprolol and atenolol standards, the instruments used are as described above, and the separation and detection steps are as follows:

[0056] 1. Preparation of standard solutions of metoprolol and atenolol

[0057] The stock solution concentration of metoprolol and atenolol was 1.0mmol / L. The stock solution was stored in a refrigerator at 4°C, protected from light.

[0058] 2.10.0mmol / L, NaH at pH3.0 2 PO 4 -Na 2 HPO 4 Preparation of buffer solution

[0059] First prepare NaH with a concentration of 10.0mmol / L 2 PO 4 Solution and H at a concentration of 1.0mmol / L 3 PO 4 solution, followed by H 3 PO 4 The solution was adjusted to pH 3.0.

[0060] 3.100.0mmol / L, NaH at pH8.5 2 PO 4 -Na 2 HPO 4 Preparation of buffer solution

[0061] First prepare NaH with a concentration of 100.0mmol / L 2 PO 4 solution and a co...

Embodiment 3

[0067] Example 3 Separation and detection of metoprolol and atenolol in urine samples

[0068] For the separation and detection of metoprolol and atenolol in urine samples, the instruments used are as described above, and the separation and detection steps are as follows:

[0069] 1.10.0mmol / L, NaH at pH3.0 2 PO 4 -Na 2 HPO 4Preparation of buffer solution

[0070] First prepare NaH with a concentration of 10.0mmol / L 2 PO 4 Solution and H at a concentration of 1.0mmol / L 3 PO 4 solution, followed by H 3 PO 4 The solution was adjusted to pH 3.0.

[0071] 2.100.0mmol / L, NaH at pH8.5 2 PO 4 -Na 2 HPO 4 Preparation of buffer solution

[0072] First prepare NaH with a concentration of 100.0mmol / L 2 PO 4 solution and a concentration of 100.0mmol / L Na 2 HPO 4 solution, and then mix the two to adjust the pH to 8.5.

[0073] 3. The preparation process of the blank urine sample and standard urine samples spiked with metoprolol and atenolol is as follows:

[0074] Take...

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Abstract

The invention relates to electrocapillarity electrophoresis electrochemiluminescence inspection. It makes direct analysis for the standard sample solution and marked urine sample. It has high sensitivity and simple operation. The inspection linear scope for two standard solutions are 5*10-8-1. 0*10-5mol/L and 7. 5*10-8-1. 0*10-6mol/L, lower limit 5. 0*10-9mol/L and 7. 5*10-8mol/L. It can also be used for betaantagonist analysis with far reaching significance in betaantagonist clinical inspection.

Description

technical field [0001] The invention belongs to the technical field of electrochemiluminescence detection by capillary electrophoresis, in particular to a method for detecting metoprolol and atenolol by electrochemiluminescence by capillary electrophoresis. Background technique [0002] Metoprolol and atenolol belong to the class of β(1)-adrenergic receptor inhibitor drugs, which are widely used in the treatment of cardiovascular diseases such as hypertension, angina pectoris, arrhythmia and myocardial infarction. It is also used as a stimulant in sports due to its sedative effects. Overdose of these drugs can lead to slow heartbeat, hypotension, bronchospasm and hypoglycemia. Therefore, the development of rapid and sensitive analytical methods for the detection of such drugs is of great significance in clinical testing, doping control and pharmacokinetic research. [0003] At present, the detection of such drugs mainly uses the method of chromatography-mass spectrometry, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N27/447G01N27/48
Inventor 由天艳黄建设
Owner CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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