Compositions and methods for detecting and quantifying COX-2 activity and anandamide metabolites
a technology of cox-2 and anandamide, applied in the field of cyclooxygenases, can solve the problems of invasive nature and expense of pet equipment, the method only localizes cox-2 protein, and does not detect or measure activity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
example 4
5.4 Example 4
[0102] COX-2 expression and activity are generally linked with the inflammatory process, which accompanies a plethora of pathologies including, but not limited to, arthritis / arthropathy, infectious disease, neurodegenerative disease, neoplasia and autoimmune disease. The quantification of prostaglandin PGH.sub.2-EA metabolites from biological fluids obtained non-invasively (e.g., blood, urine) will allow for the assessment of COX-2 activity in vivo, reflecting both inflammation and disease severity. In addition, serial testing will allow for the tracking of the natural course of the disease as well as the efficacy of anti-inflammatory therapy. A model for this application would be the ubiquitous use of C-reactive protein (CRP) in the diagnosis and assessment of diseases associated with inflammation. The benefits of using PGH.sub.2-EA metabolites in this context instead of more traditional diagnostic markers, such as CRP, involve the highly specific nature of PGH.sub.2-E...
example 5
5.5 Example 5
[0104] COX-2 expression and activity are linked to several solid tumors, most notably colorectal adenocarcinoma. The quantification of PGH.sub.2-EA metabolites from biological fluids, described herein, provides a noninvasive "early-warning" for clinically undetectable neoplasia. In addition, serial testing following diagnosis will allow for the tracking of the natural course of the cancer as well as the efficacy of it) antineoplastic therapy. A model for this application would be the use of prostate specific antigen (PSA) in the diagnosis and assessment of prostate adenocarcinomas. The benefits of PGH.sub.2-EA metabolite quantification in this context include (a) relative noninvasiveness, (b) sensitivity (most cancers are advanced once symptomatic) and (c) cost (simple lab diagnostic technique versus colonoscopy for example).
[0105] Scenario: Elderly asymptomatic man receives annual physical examination. Urine and blood are collected and PGH.sub.2-EA metabolite quantific...
example 6
5.6 Example 6
[0106] The measurement of PGH.sub.2-EA metabolites from in vitro samples (e.g., cell culture, biopsy samples) allows for the direct quantification of COX-2 activity. Current methods which quantify cyclooxygenase activity do not directly distinguish between COX-1 and COX-2. Methods which quantify COX-2 expression (e.g., Western blotting) do not assess activity which may or may not correlate with expression levels.
[0107] Scenario: Researchers investigating new NSAIDS expose cultured cells M) expressing COX-2 to various concentrations of test compounds for predetermined periods of time. At the conclusion of the exposures, conditioned medium is collected from each culture. The samples of conditioned medium are assayed for the presence of PGH.sub.2-EA metabolite. The researchers find that most of the test compounds have no significant affect on the production of PGH.sub.2-EA metabolite by the cultured cells. However, one compound ("compound X") dramatically reduces the amoun...
PUM
| Property | Measurement | Unit |
|---|---|---|
| apparent volume of distribution | aaaaa | aaaaa |
| capillary temperature | aaaaa | aaaaa |
| apparent volume of distribution | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More