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Polynucleotides encoding thrombin receptor homologs

Inactive Publication Date: 2002-09-12
INCYTE PHARMA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

High blood pressure within the liver is a significant factor in edema or ascites.

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  • Polynucleotides encoding thrombin receptor homologs
  • Polynucleotides encoding thrombin receptor homologs
  • Polynucleotides encoding thrombin receptor homologs

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[0062] I Isolation of mRNA and Construction of the cDNA Library

[0063] The TRH sequence of this application was first identified in Incyte clone No. 86700 among the sequences comprising the human liver library. The human cells used for this library came from a 49 year old human male (Catalogue # 937224; Stratagene, LaJolla Calif.). cDNA synthesis for the cDNA library was primed with oligo dT, and synthetic adaptor oligonucleotides were ligated onto cDNA ends enabling its insertion into UNIZAP vector system (Stratagene). This allowed high efficiency unidirectional (sense orientation) lambda library construction and the convenience of a plasmid system with blue / white color selection to detect clones with cDNA insertions.

[0064] The cDNA library can be screened with either DNA probes or antibody probes and the PBLUESCRIPT phagemid (Stratagene) can be rapidly excised in vivo. The phagemid allows the use of a plasmid system for easy insert characterization, sequencing, site-directed mutage...

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Abstract

The present invention provides nucleotide and amino acid sequences that identify and encode a novel thrombin receptor homolog (TRH) expressed in human liver. The present invention also provides for antisense molecules to the nucleotide sequences which encode TRH, diagnostic tests based on TRH encoding nucleic acid molecules, expression vectors for the production of purified TRH, antibodies capable of binding specifically to TRH, hybridization probes or oligonucleotides for the detection of TRH-encoding nucleotide sequences, genetically engineered host cells for the expression of TRH, and antagonists, antibodies and inhibitors with specific binding activity for the polypeptide TRH.

Description

[0001] This application is a continuation application of U.S. application Ser. No. 09 / 643,383, filed Aug. 21, 2000, entitled POLYNUCLEOTIDES ENCODING THROMBIN RECEPTOR HOMOLOGS (as amended), which is a divisional application of U.S. application Ser. No. 09 / 217,101, filed Dec. 21, 1998 and issued on Nov. 7, 2000 as U.S. Pat. No. 6,143,870, entitled THROMBIN RECEPTOR HOMOLOG, which is a divisional application of U.S. application Ser. No. 08 / 911,320, filed Aug. 14, 1997 and issued on Feb. 9, 1999 as U.S. Pat. No. 5,869,633, entitled THROMBIN RECEPTOR HOMOLOG POLYNUCLEOTIDE, which is a divisional application of U.S. application Ser. No. 08 / 467,125, filed Jun. 6, 1995 and issued on Nov. 11, 1997 as U.S. Pat. No. 5,686,597, entitled THROMBIN RECEPTOR HOMOLOG, the contents all of which are hereby incorporated by reference.FIELD OF THE INVENTION[0002] This invention relates to nucleic acid and amino acid sequences of a new human thrombin receptor homolog and the use thereof fro the diagnosi...

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Application Information

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IPC IPC(8): A61K31/70A61K38/00A61K39/395A61K48/00A61P43/00C07K14/705C12N15/09C12N15/12C12P21/02C12Q1/68G01N33/53
CPCA61K38/00A61K48/00C07K14/705C07K2319/00A61P43/00
Inventor COLEMAN, ROGERAU-YOUNG, JANICEBANDMAN, OLGASEILHAMER, JEFFREY J.
Owner INCYTE PHARMA INC